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Expression of MRJP3 and HSP70 mRNA Levels in Apis mellifera L. Workers after Dietary Supplementation with Proteins, Prebiotics, and Probiotics.
Oliveira, Maria Carolina Paleari Varjão; Pereira, Eloisa Magalhaes; Sereia, Maria Josiane; Lima, Érica Gomes; Silva, Breno Gabriel; Toledo, Vagner Alencar Arnaut; Ruvolo-Takasusuki, Maria Claudia Colla.
Afiliação
  • Oliveira MCPV; Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá CEP 87020-900, Brazil.
  • Pereira EM; Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá CEP 87020-900, Brazil.
  • Sereia MJ; Department of Engineering and Food Technology, Federal Technological University of Paraná, Campo Mourão CEP 87301-899, Brazil.
  • Lima ÉG; Department of Animal Science, Federal University of Sergipe, Aracaju CEP 49100-000, Brazil.
  • Silva BG; Department of Exact Sciences, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba CEP 13418-900, Brazil.
  • Toledo VAA; Department of Animal Science, State University of Maringá, Maringá CEP 87020-900, Brazil.
  • Ruvolo-Takasusuki MCC; Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, Maringá CEP 87020-900, Brazil.
Insects ; 13(7)2022 Jun 24.
Article em En | MEDLINE | ID: mdl-35886747
Royal jelly is an essential substance for the development of bees from larval to adult stages. Studies have identified a group of key proteins in royal jelly, denominated major royal jelly proteins (MRJPs). The group currently consists of nine proteins (MRJP1-MRJP9), with MRJP1 being the most abundant and MRJP3 being used as a microsatellite marker for the selection of queens with a greater production of royal jelly. The diet of bees is mostly composed of proteins, and supplementing this intake to encourage a higher production of their primary product is important for producers. It is estimated that, by adding probiotic and prebiotic organisms to their diets, the benefits to bees will be even greater, both for their immune systems and primary responses to stress. Circumstances that are adverse compared to those of the natural habitat of bees eventually substantially interfere with bee behavior. Stress situations are modulated by proteins termed heat shock proteins (HSPs). Among these, HSP70 has been shown to exhibit abundance changes whenever bees experience unusual situations of stress. Thus, we sought to supplement A. mellifera bee colony diets with proteins and prebiotic and probiotic components, and to evaluate the expression levels of MRJP3 and HSP70 mRNAs using qRT-PCR. The results revealed that differences in the expression of MRJP3 can be attributed to the different types of feed offered. Significant differences were evident when comparing the expression levels of MRJP3 and HSP70, suggesting that protein supplementation with pre/probiotics promotes positive results in royal jelly synthesis carried out by honey bee nurses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Insects Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Insects Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça