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Development of a Rapid Gold Nanoparticle-Based Lateral Flow Immunoassay for the Detection of Dengue Virus.
Martinez-Liu, Cynthia; Machain-Williams, Carlos; Martinez-Acuña, Natalia; Lozano-Sepulveda, Sonia; Galan-Huerta, Kame; Arellanos-Soto, Daniel; Meléndez-Villanueva, Mayra; Ávalos-Nolazco, Diana; Pérez-Ibarra, Katya; Galindo-Rodríguez, Sergio; de Jesús Garza-Juarez, Aurora; Rivas-Estilla, Ana María.
Afiliação
  • Martinez-Liu C; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
  • Machain-Williams C; Laboratory of Arbovirology, Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatan, Merida 97000, Yucatan, Mexico.
  • Martinez-Acuña N; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
  • Lozano-Sepulveda S; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
  • Galan-Huerta K; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
  • Arellanos-Soto D; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
  • Meléndez-Villanueva M; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
  • Ávalos-Nolazco D; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
  • Pérez-Ibarra K; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
  • Galindo-Rodríguez S; Department of Chemistry, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Monterrey 66450, Nuevo León, Mexico.
  • de Jesús Garza-Juarez A; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
  • Rivas-Estilla AM; Department of Biochemistry and Molecular Medicine, Hospital Universitario "Dr. Jose E. Gonzalez", Autonomous University of Nuevo Leon, Monterrey 64460, Nuevo León, Mexico.
Biosensors (Basel) ; 12(7)2022 Jul 07.
Article em En | MEDLINE | ID: mdl-35884298
Flavivirus detection in humans and mosquito reservoirs has been an important issue since it can cause a variety of illnesses and could represent a health problem in geographical zones where the vector is endemic. In this work, we designed and characterized a biosensor based on gold nanoparticles (AuNPs) and antibody 4G2 for the detection of dengue virus (DENV) in vitro, obtaining different conjugates (with different antibody concentrations). The AuNP-4G2 conjugates at concentrations of 1, 3, and 6 µg/mL presented an increase in the average hydrodynamic diameter compared to the naked AuNPs. Also, as part of the characterization, differences in the UV-Vis absorbance spectrum and electrophoretic migration were observed between the conjugated AuNPs (with BSA or antibody) and naked AuNPs. Additionally, we used this biosensor (AuNP-4G2 conjugate with 3 µg/mL antibody) in the assembly of a competitive lateral flow assay (LFA) for the development of an alternative test to detect the flavivirus envelope protein in isolated DENV samples as a future tool for dengue detection (and other flaviviruses) in the mosquito vector (Aedesaegypti) for the identification of epidemic risk regions. Functionality tests were performed using Dengue virus 2 isolated solution (TCID50/mL = 4.58 × 103) as a positive sample and PBS buffer as a negative control. The results showed that it is possible to detect Dengue virus in vitro with this gold nanoparticle-based lateral flow assay with an estimated detection limit of 5.12 × 102 PFU. We suggest that this biosensor could be used as an additional detection tool by coupling it to different point-of-care tests (POCT) for the easy detection of other flaviviruses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Vírus da Dengue / Nanopartículas Metálicas Tipo de estudo: Diagnostic_studies Limite: Animals / Humans Idioma: En Revista: Biosensors (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Vírus da Dengue / Nanopartículas Metálicas Tipo de estudo: Diagnostic_studies Limite: Animals / Humans Idioma: En Revista: Biosensors (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México País de publicação: Suíça