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Homoscleromorpha-derived Bacillus spp. as potential sources of biotechnologically-relevant hydrolases and biosurfactants.
de Oliveira, Thiago Silva; de Oliveira, Bruno Francesco Rodrigues; de Andrade, Flavia Costa Carvalho; Guimarães, Carolina Reis; de Godoy, Mateus Gomes; Laport, Marinella Silva.
Afiliação
  • de Oliveira TS; Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, 21941-902, Brazil.
  • de Oliveira BFR; Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, 21941-902, Brazil.
  • de Andrade FCC; Instituto Biomédico, Universidade Federal Fluminense, Rua Professor Hernani Melo, 101, São Domingos, Niterói, RJ, 24210-130, Brazil.
  • Guimarães CR; Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, 21941-902, Brazil.
  • de Godoy MG; Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, 21941-902, Brazil.
  • Laport MS; Instituto de Química, Universidade Federal do Rio de Janeiro, Av. Athos da Silveira Ramos, 149, Cidade Universitária, Rio de Janeiro, 21941-909, Brazil.
World J Microbiol Biotechnol ; 38(10): 169, 2022 Jul 27.
Article em En | MEDLINE | ID: mdl-35882683
Despite hydrolytic exoenzymes and biosurfactants having been gradually reported from the poriferan microbiome, little is known about these bioproducts in microorganisms inhabiting Homoscleromorpha sponges. Here, we investigated the production of hydrolases and biosurfactants in bacteria isolated from three shallow-water homoscleromorph species, Oscarella sp., Plakina cyanorosea, and Plakina cabofriense. A total of 99 of 107 sponge-associated bacterial isolates exhibited activity for at least one of the analyzed hydrolases. Following fermentation in Luria-Bertani (LB) and Tryptic Soy Broth (TSB), two isolates, 80BH11 and 80B1:1010b, showed higher lipase and peptidase activities. Both of them belonged to the Bacillus genus and were isolated from Oscarella. Central composite design leveraged up the peptidase activity in 280% by Bacillus sp. 80BH11 in the TSB medium for 48 h at 30 °C. The optimized model also revealed that pH 6.5 and 45 °C were the best conditions for peptidase reaction. In addition, Bacillus sp. 80BH11 was able to release highly emulsifying and remarkably stable surfactants in the LB medium. Surfactin was finally elucidated as the biosurfactant generated by this sponge-derived Bacillus. In conclusion, we hope to have set the scenery for further prospecting of industrial enzymes and biosurfactants in Homoscleromorpha microbiomes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poríferos / Bacillus Limite: Animals Idioma: En Revista: World J Microbiol Biotechnol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poríferos / Bacillus Limite: Animals Idioma: En Revista: World J Microbiol Biotechnol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha