Your browser doesn't support javascript.
loading
Pequi oil (Caryocar brasilense Cambess.) nanoemulsion alters cell proliferation and damages key organelles in triple-negative breast cancer cells in vitro.
Ombredane, Alicia S; Silva, Lídia R A; Araujo, Victor H S; Costa, Patrícia L; Silva, Lucas C; Sampaio, Marina C; Lima, Milena C F; Veiga Junior, Valdir F; Vieira, Ivo J C; Azevedo, Ricardo B; Joanitti, Graziella A.
Afiliação
  • Ombredane AS; Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano), University of Brasilia, Campus Universitário - Centro Metropolitano, Ceilândia Sul, Brasília, DF 72220-275, Brazil; Post-Graduation Program in Nanoscience and Nanobiotechnology, Institute of Biological Sciences, University of Brasili
  • Silva LRA; Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano), University of Brasilia, Campus Universitário - Centro Metropolitano, Ceilândia Sul, Brasília, DF 72220-275, Brazil.
  • Araujo VHS; Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano), University of Brasilia, Campus Universitário - Centro Metropolitano, Ceilândia Sul, Brasília, DF 72220-275, Brazil.
  • Costa PL; Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano), University of Brasilia, Campus Universitário - Centro Metropolitano, Ceilândia Sul, Brasília, DF 72220-275, Brazil; Post-Graduation Program in Sciences and Technologies in Health, Faculty of Ceilandia, University of Brasilia, Campus
  • Silva LC; Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano), University of Brasilia, Campus Universitário - Centro Metropolitano, Ceilândia Sul, Brasília, DF 72220-275, Brazil; Post-Graduation Program in Sciences and Technologies in Health, Faculty of Ceilandia, University of Brasilia, Campus
  • Sampaio MC; Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano), University of Brasilia, Campus Universitário - Centro Metropolitano, Ceilândia Sul, Brasília, DF 72220-275, Brazil; Post-Graduation Program in Nanoscience and Nanobiotechnology, Institute of Biological Sciences, University of Brasili
  • Lima MCF; Chemistry Section, Military Institute of Engineering, Praça Gen. Tibúrcio, 80, Praia Vermelha, Rio de Janeiro, RJ 22290-270, Brazil.
  • Veiga Junior VF; Chemistry Section, Military Institute of Engineering, Praça Gen. Tibúrcio, 80, Praia Vermelha, Rio de Janeiro, RJ 22290-270, Brazil.
  • Vieira IJC; Laboratório de Ciências Químicas-LCQUI, Universidade Estadual do Norte Fluminense Darcy Ribeiro-UENF, Avenida Alberto Lamego 2000, Campos dos Goytacazes, RJ 28013-602, Brazil.
  • Azevedo RB; Post-Graduation Program in Nanoscience and Nanobiotechnology, Institute of Biological Sciences, University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília 70910-900, Brazil; Laboratory of Nanobiotechnology, Institute of Biological Sciences, Department of Genetics and Morphology, University
  • Joanitti GA; Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano), University of Brasilia, Campus Universitário - Centro Metropolitano, Ceilândia Sul, Brasília, DF 72220-275, Brazil; Post-Graduation Program in Nanoscience and Nanobiotechnology, Institute of Biological Sciences, University of Brasili
Biomed Pharmacother ; 153: 113348, 2022 Sep.
Article em En | MEDLINE | ID: mdl-35820315
Pequi oil is extracted from the fruit of a Brazilian native plant (Caryocar brasiliense Camb) that contains some molecules with anticancer potential. Due to its hydrophobic property, the administration of pequi oil associated with nanoemulsion systems represents a successful strategy to improve oil bioavailability. Breast cancer is the most frequent type of cancer among women and conventional therapies used are frequently associated with several side effects. Thus, the aim of this study was to investigate the effects of pequi oil-based nanoemulsion (PeNE) on triple-negative breast cancer cells (4T1), in vitro. PeNE presented a dose- and time-dependent cytotoxic effect with lower IC50 than free pequi oil after 48 h of exposure (p < 0.001). At 180 µg/mL, PeNE demonstrated numerous cell alterations, when compared to free pequi oil, such as morphological alterations, reduction in cell proliferation and total cell number, damage to plasmatic membrane, induction of lysosomal membrane permeability and depolarization of mitochondrial membrane, alteration of intracellular ROS production and calcium level, and increase in phosphatidylserine exposure. Taken together, the results suggest an interesting induction of cell death mechanisms involving a combined action of factors that impair nucleus, mitochondria, lysosome, and ER function. In addition, more pronounced effects were observed in cells treated by PeNE at 180 µg/mL when compared to free pequi oil, thereby reinforcing the advantages of using nanometric platforms. These promising results highlight the use of PeNE as a potential complementary therapeutic approach to be employed along with conventional treatments against breast cancer in the future.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ericales / Neoplasias de Mama Triplo Negativas / Malpighiales Limite: Female / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2022 Tipo de documento: Article País de publicação: França
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ericales / Neoplasias de Mama Triplo Negativas / Malpighiales Limite: Female / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2022 Tipo de documento: Article País de publicação: França