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Increased ß-hydroxybutyrate (BHBA) concentration affect follicular growth in cattle.
Missio, Daniele; Fritzen, Alexandro; Cupper Vieira, Camila; Germano Ferst, Juliana; Farias Fiorenza, Mariani; Guedes de Andrade, Leonardo; Martins de Menezes, Bento; Tomazele Rovani, Monique; Gazieira Gasperin, Bernardo; Dias Gonçalves, Paulo Bayard; Ferreira, Rogério.
Afiliação
  • Missio D; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Fritzen A; Department of Animal Science, Santa Catarina State University, Chapecó, SC, Brazil.
  • Cupper Vieira C; Molecular and Integrative Physiology of Reproduction Laboratory, MINT, Federal University of Pampa, Uruguaiana, RS, Brazil.
  • Germano Ferst J; Molecular and Integrative Physiology of Reproduction Laboratory, MINT, Federal University of Pampa, Uruguaiana, RS, Brazil.
  • Farias Fiorenza M; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Guedes de Andrade L; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Martins de Menezes B; Molecular and Integrative Physiology of Reproduction Laboratory, MINT, Federal University of Pampa, Uruguaiana, RS, Brazil.
  • Tomazele Rovani M; Department of Animal Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Gazieira Gasperin B; Department of Animal Pathology, Federal University of Pelotas, Capão do Leão, RS, Brazil.
  • Dias Gonçalves PB; Molecular and Integrative Physiology of Reproduction Laboratory, MINT, Federal University of Pampa, Uruguaiana, RS, Brazil.
  • Ferreira R; Department of Animal Science, Santa Catarina State University, Chapecó, SC, Brazil. Electronic address: rogerio.ferreira@udesc.br.
Anim Reprod Sci ; 243: 107033, 2022 Aug.
Article em En | MEDLINE | ID: mdl-35816934
Metabolic stress conditions caused by negative energy balance (NEB) have been associated with reduced fertility in cows. ß-hydroxybutyrate (BHBA) is the main circulating ketone body, which accumulates within follicular fluid. The aim of this study was to evaluate the effects of BHBA on follicle growth and on ovulatory mechanisms in cattle. At 72 h after intrafollicular injection, there was a decrease in follicular diameter in BHBA group compared to control (P = 0.02). Furthermore, follicle growth rate was reduced post-treatment with BHBA in comparison to the control group (P < 0.03). The BHBA intrafollicular injection in follicles ≥ 12 mm, however, did not affect E2 and P4 concentrations in the follicular fluid. In addition, the relative abundance of genes involved in the ovulatory cascade (ADAM 17, AREG, EREG, PTGS2), steroidogenesis (CYP19A1, 3BHSD, STAR), cellular stress (SOD1, CAT, GPX1, HSPA5, XBP1s, XBP1u, ATF4, ATF6), monocarboxylic acid transporters (SLC16A1, SLC16A7) and apoptosis (XIAP) was similar between groups. In conclusion, the results of this study indicate that the increase in intrafollicular concentrations of BHBA affects follicular growth, but it does not compromise the ovulatory cascade and cellular homeostasis in bovine granulosa cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Folículo Ovariano / Células da Granulosa Limite: Animals Idioma: En Revista: Anim Reprod Sci Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Folículo Ovariano / Células da Granulosa Limite: Animals Idioma: En Revista: Anim Reprod Sci Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda