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Specific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma.
Vieira, Paula; Jesus, Viviane; Cândido, Marcela Aparecida; Pacheco-Soares, Cristina; Castilho, Maiara; Raniero, Leandro.
Afiliação
  • Vieira P; Nanosensors Laboratory, Research & Development Institute, Vale do Paraíba University, 12244-000 São José dos Campos, São Paulo, Brazil.
  • Jesus V; Nanosensors Laboratory, Research & Development Institute, Vale do Paraíba University, 12244-000 São José dos Campos, São Paulo, Brazil.
  • Cândido MA; Nanosensors Laboratory, Research & Development Institute, Vale do Paraíba University, 12244-000 São José dos Campos, São Paulo, Brazil.
  • Pacheco-Soares C; Dynamics of Cellular Compartments Laboratory, Research & Development Institute, Vale do Paraíba University, 12244-000 São José dos Campos, São Paulo, Brazil. Electronic address: cpsoares@univap.br.
  • Castilho M; Bionanotechnology Laboratory, Research & Development Institute, Vale do Paraíba University, 12244-000 São José dos Campos, São Paulo, Brazil. Electronic address: mcastilho@univap.br.
  • Raniero L; Nanosensors Laboratory, Research & Development Institute, Vale do Paraíba University, 12244-000 São José dos Campos, São Paulo, Brazil. Electronic address: lraniero@univap.br.
Photodiagnosis Photodyn Ther ; 39: 102997, 2022 Sep.
Article em En | MEDLINE | ID: mdl-35781094
BACKGROUND: Epidermal Growth Factor Receptor (EGFR receptor) is encoded by the EGFR gene. EGFR receptor signaling pathways are activated by EGF protein, regulating cell actions. Overexpression of EGFR receptor may be linked to malignancies with a poor prognosis. As a result, EGFR receptor is being studied for a variety of tumor diagnostics, spurring the development of innovative approaches to increase quality and efficiency. Nanomaterials can recognize cancer cells by specifically targeting of molecular pathways, underscoring the importance of nanomedicine. In this study, we synthesized EGFR-specific nanomarkers by functionalizing EGF protein and Chlorin e6 in gold nanoparticles. These nanoparticles use active targeting to deliver EGF protein to EGFR receptor, and Chlorin e6 serves as a fluorescent marker molecule METHODS: Nanomarkers were examined in vitro in MDA-MB-468 and M059J cell lines. Confocal microscopy and flow cytometry were used to examine the distribution, uptake, internalization, and fluorescence intensity of nanomarkers in vitro RESULTS: The results show that both lines examined accumulate nanomarkers. However, MDA-MB-468 had the highest intensity due to its EGFR receptor overexpression properties CONCLUSION: The findings point to ideal properties for detecting EGFR receptor overexpressed cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Neoplasias da Mama / Glioblastoma / Nanopartículas Metálicas / Neoplasias de Mama Triplo Negativas Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Assunto da revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Neoplasias da Mama / Glioblastoma / Nanopartículas Metálicas / Neoplasias de Mama Triplo Negativas Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Assunto da revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda