Human hookworms from Argentina: Differential diagnosis of Necator americanus and Ancylostoma duodenale in endemic populations from Buenos Aires and Misiones.

Servián, Andrea; Repetto, Silvia A; Lorena Zonta, María; Navone, Graciela T

Servián, Andrea; Repetto, Silvia A; Lorena Zonta, María; Navone, Graciela T.

Afiliação

Servián A; Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CONICET-UNLP), La Plata, Buenos Aires, Argentina. Electronic address: servianandrea3@gmail.com.
Repetto SA; Instituto de Investigación en Microbiología y Parasitología Médica (IMPaM), Facultad de Medicina, Universidad de Buenos Aires - CONICET, Ciudad Autónoma de Buenos Aires, Argentina.
Lorena Zonta M; Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CONICET-UNLP), La Plata, Buenos Aires, Argentina.
Navone GT; Centro de Estudios Parasitológicos y de Vectores (CEPAVE-CONICET-UNLP), La Plata, Buenos Aires, Argentina.

Rev Argent Microbiol ; 54(4): 268-281, 2022.

Article em En | MEDLINE | ID: mdl-35725664

RESUMO

Hookworm infection is endemic in many countries throughout the world; however, the information about the prevalence of each species, Necatoramericanus and Ancylostomaduodenale, is inaccurate in many South American countries. We aimed to determine the prevalence of human hookworm species by combining the results of both microscopy and PCR among endemic populations in Argentina, represented by natives and immigrants. A total of 140 serial fecal specimens were obtained from natives in the province of Misiones and an immigrant community living in the province of Buenos Aires. Samples were examined using the formalin-ethyl acetate concentration technique (FECT) and one flotation technique (screening tests) and specific PCRs for N. americanus and A. duodenale. We characterized samples containing N. americanus by sequencing a fragment of the cytochrome b gene. The observed hookworm prevalence as assessed by the screening tests and PCR were 24.3% and 32.8%, respectively. PCR positive samples were identified as N. americanus. PCR had 100% sensitivity compared with 73.9% of screening tests. A total of 12 samples from individuals with hookworm-infected household members were positive only by PCR. N. americanus sequences showed 90.5% identity, being more similar to each other than to any of the sequences obtained from GenBank. This is the first study that provides molecular data and characterization of N. americanus in Argentina. The complementary use of FECT and one flotation technique to screen hookworm infections, followed by PCR to differentiate the species contribute to produce better prevalence estimates.

Assuntos

Ancylostoma; Necator americanus; Animais; Humanos; Ancylostoma/genética; Necator americanus/genética; Ancylostomatoidea; Diagnóstico Diferencial; Argentina/epidemiologia; Fezes

Palavras-chave

Ancylostoma duodenale; Diagnóstico diferencial; Differential diagnosis; Examinación microscópica; Microscopic examination; Necator americanus; PCR

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Necator americanus / Ancylostoma Tipo de estudo: Diagnostic_studies / Risk_factors_studies Limite: Animals / Humans País/Região como assunto: America do sul / Argentina Idioma: En Revista: Rev Argent Microbiol Ano de publicação: 2022 Tipo de documento: Article País de publicação: Argentina

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