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Protective Effectiveness of an Immunization Protocol Against the Toxic Effects of Loxosceles intermedia Venom in Rabbits.
de Miranda, Ana Luísa Soares; Lima, Sabrina de Almeida; Botelho, Ana Flávia Machado; Gomes Campos, Marco Túlio; Eckstein, Camila; Minozzo, João Carlos; Chávez-Olórtegui, Carlos Delfin; Soto-Blanco, Benito.
Afiliação
  • de Miranda ALS; Department of Veterinary Clinics and Surgery, Veterinary College, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Lima SA; Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Botelho AFM; Department of Veterinary Medicine, Veterinary College, Federal University of Goiás, Goiânia, Brazil.
  • Gomes Campos MT; Department of Veterinary Clinics and Surgery, Veterinary College, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Eckstein C; Department of Veterinary Clinics and Surgery, Veterinary College, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Minozzo JC; Department of Health of the State of Paraná, Production and Research Center of Immunobiologicals, Piraquara, Brazil.
  • Chávez-Olórtegui CD; Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Soto-Blanco B; Department of Veterinary Clinics and Surgery, Veterinary College, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Front Vet Sci ; 9: 852917, 2022.
Article em En | MEDLINE | ID: mdl-35711800
Loxosceles spp. (brown spiders) bites are responsible for the development of a syndrome consisting mainly of dermonecrotic lesions, and also systemic effects. Rabbits are one of the main experimental models used for better understanding the systemic and local effects of Loxosceles venom. The aim of this study is to evaluate the toxic and protective effects of rabbits immunized with Loxosceles spp. venom. Male New Zealand rabbits were allocated as a control group (CG; n = 5) that received adjuvant (Montanide) and phosphate-buffer saline (PBS), or as venom group (VG; n = 5) that received 21 µg of Loxosceles venom using Montanide as adjuvant. After five immunization cycles, a trial with 7 µg of Loxosceles intermedia (L. intermedia) venom was performed, and dermonecrotic lesions were measured. The rabbits were then euthanized, and their organs were collected for histopathology analysis. Rabbits that had undergone Loxosceles venom immunization protocol showed minor clinical disturbances during the experimental period. The used immunization protocol protected the rabbits against the toxic effect of the Loxosceles venom because they showed minor clinical disturbances during the experimental period.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Guideline Idioma: En Revista: Front Vet Sci Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Guideline Idioma: En Revista: Front Vet Sci Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça