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Molecular diagnosis of trypanosomatids in Didelphis marsupialis from Los Montes de María: a first report of Trypanosoma rangeli from Colombian Caribbean region.
Ardila, Marlon Mauricio; Herrera, Leidi; Zabala-Monterroza, Wendy; Bedoya-Polo, Alexander; Lozano-Arias, Daisy; García-Alzate, Roberto; Pérez-Doria, Alveiro.
Afiliação
  • Ardila MM; Grupo Interdisciplinario en Ciencias Marinas y Ambientales (GICMARA), Facultad de Ciencias Básicas, Universidad del Atlántico, Puerto Colombia, Colombia.
  • Herrera L; Grupo de Investigación Básica y Clínica en Ciencias de La Salud, Facultad de Ciencias de la Salud, Fundación Universitaria San Martín, Puerto Colombia, Colombia.
  • Zabala-Monterroza W; Laboratorio de Biología de Vectores y Parásitos, Facultad de Ciencias, Instituto de Zoología Y Ecología Tropical, Universidad Central de Venezuela, Caracas, Venezuela.
  • Bedoya-Polo A; Grupo de Investigaciones Biomédicas, Laboratorio de Biomédicas, Universidad de Sucre, Sincelejo, Colombia.
  • Lozano-Arias D; Grupo de Investigaciones Biomédicas, Laboratorio de Biomédicas, Universidad de Sucre, Sincelejo, Colombia.
  • García-Alzate R; Grupo de Investigación Básica y Clínica en Ciencias de La Salud, Facultad de Ciencias de la Salud, Fundación Universitaria San Martín, Puerto Colombia, Colombia.
  • Pérez-Doria A; Programa de Biología, Facultad de Ciencias Básicas, Universidad del Atlántico, Puerto Colombia, Colombia.
J Parasit Dis ; 46(2): 323-327, 2022 Jun.
Article em En | MEDLINE | ID: mdl-35692475
Didelphis marsupialis is a primary reservoir of Trypanosoma cruzi, etiologic agent of American Trypanosomiasis-AT or Chagas Disease-CD, in America. Some findings of Trypanosoma rangeli have been recorded in this mammal, in sympatry with T. cruzi. In Los Montes de María, Bolívar, Colombian Caribbean, triatomine insects and potential parasite host has been registered, but little is known about the relationship between these parasites and D. marsupialis. We investigated the natural trypanosomatids infection rate in D. marsupialis, applying a parasitological and molecular diagnosis. Twenty D. marsupialis was investigated between 2018 and 2019 using 21 Tomahawk® traps placed on the sylvatic/domestic corridors. Blood was drawn by cardiopuncture after sedation. An aliquot of blood samples was cultured in Novy, Nicolle, McNeal/Roswell Park Memorial Institute medium at 24 °C/60 days for the detection of motile trypomastigotes. Parasite DNA was obtained by salting out methods from positive blood cultures. Trypanosomatids diagnosis was done by Polymerase Chain Reaction-sequencing of V7V8 region of 18S ribosomal RNA (18S-rRNA) gene. Amplicons were sequenced, and consensus sequences were aligned with reference sequences from GenBank. Four isolates corresponded to T. rangeli (20%) and one to T. cruzi (5%). The natural infection of D. marsupialis by T. rangeli and T. cruzi constitutes the first record of these parasites in didelphids in Los Montes de María and the first record of T. rangeli in this marsupial, in the Colombian Caribbean.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies País/Região como assunto: America do sul / Colombia Idioma: En Revista: J Parasit Dis Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Índia
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies País/Região como assunto: America do sul / Colombia Idioma: En Revista: J Parasit Dis Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Índia