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Whole genome analysis of UV-induced mutagenesis in Caulobacter crescentus.
Alves, Ingrid R; Vêncio, Ricardo Z; Galhardo, Rodrigo S.
Afiliação
  • Alves IR; Institute of Biomedical Sciences, Department of Microbiology, University of São Paulo, São Paulo, Brazil.
  • Vêncio RZ; Department of Computing and Mathematics FFCLRP, Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
  • Galhardo RS; Institute of Biomedical Sciences, Department of Microbiology, University of São Paulo, São Paulo, Brazil. Electronic address: rgalhard@usp.br.
Mutat Res ; 825: 111787, 2022.
Article em En | MEDLINE | ID: mdl-35691139
UV-induced mutagenesis is, to greater extent, a phenomenon dependent on translesion synthesis (TLS) and regulated by the SOS response in bacteria. Caulobacter crescentus, like many bacterial species, employs the ImuABC (ImuAB DnaE2) pathway in TLS. To have a better understanding of the characteristics of UV-induced mutagenesis in this organism, we performed a whole genome analysis of mutations present in survivors after an acute UVC exposure (300 J/m2). We found an average of 3.2 mutations/genome in irradiated samples, distributed in a mutational spectrum consisting exclusively of base substitutions, including tandem mutations. Although limited in conclusions by the small number of mutations identified, our study points to the feasibility of using whole-genome sequencing to study mutagenesis occurring in experiments involving a single acute exposure to genotoxic agents.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Caulobacter crescentus Tipo de estudo: Prognostic_studies Idioma: En Revista: Mutat Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Caulobacter crescentus Tipo de estudo: Prognostic_studies Idioma: En Revista: Mutat Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda