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Sustainable multifunctional phenolic lipids as potential therapeutics in Dentistry.
Dame-Teixeira, Naile; El-Gendy, Reem; Monici Silva, Isabela; Holanda, Cleonice Andrade; de Oliveira, Andressa Souza; Romeiro, Luiz Antonio Soares; Do, Thuy.
Afiliação
  • Dame-Teixeira N; Department of Dentistry, School of Health Sciences, University of Brasilia, Campus Universitário Darcy Ribeiro - UnB, Federal District, Asa Norte, Brasilia, DF, 70910-900, Brazil. nailedame@unb.br.
  • El-Gendy R; Division of Oral Biology, School of Dentistry, University of Leeds, Leeds, LS9 7TF, UK. nailedame@unb.br.
  • Monici Silva I; Division of Oral Biology, School of Dentistry, University of Leeds, Leeds, LS9 7TF, UK.
  • Holanda CA; Department of Oral Pathology, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
  • de Oliveira AS; Department of Dentistry, School of Health Sciences, University of Brasilia, Campus Universitário Darcy Ribeiro - UnB, Federal District, Asa Norte, Brasilia, DF, 70910-900, Brazil.
  • Romeiro LAS; Nucleus of Tropical Medicine, School of Medicine, University of Brasilia, Federal District, Brasilia, 70910-900, Brazil.
  • Do T; Department of Pharmacy, School of Health Sciences, University of Brasilia, Federal District, Brasilia, 70910-900, Brazil.
Sci Rep ; 12(1): 9299, 2022 06 03.
Article em En | MEDLINE | ID: mdl-35662265
Phenolic lipids components of the cashew nutshell liquid (CNSL) have molecular structures capable of chemical signalling that regulate gene expression, metabolism and inflammation. This study sets out to assess how CNSL derivatives impact oral bacteria, from an antibacterial and anti-collagenolytic perspective, as well as its biocompatibility with dental pulp stem cells. Two hemi-synthetic saturated CNSL derivative compounds were selected (LDT11-Anacardic Acids-derivative and LDT409-cardanol-derivative). Bacteriostatic activity was tested against Streptococcus mutans and Veillonella parvula. Antimicrobial capacity against preformed S. mutans biofilms was investigated using a collagen-coated Calgary Biofilm Device and confocal microscopy. Clostridium histolyticum, P. gingivalis and S. mutans biofilms were used to assess anti-collagenolytic activity. Biocompatibility with human dental pulp stromal cells (HDPSCs) was investigated (MTT for viability proportion, LDH assays for cell death rate). LDTs inhibited the bacterial growth, as well as partially inhibited bacterial collagenases in concentrations higher than 5 µg/mL. Dose-response rates of biofilm cell death was observed (LDT11 at 20, 50, 100 µg/mL = 1.0 ± 0.4, 0.7 ± 0.3, 0.6 ± 0.03, respectively). Maximum cytotoxicity was 30%. After 1 week, LDT409 had no HDPSCs death. HDPSCs viability was decreased after 24 h of treatment with LDT11 and LDT409, but recovered at 72 h and showed a massive increase in viability and proliferation after 1 week. LDTs treatment was associated with odontoblast-like morphology. In conclusion, LDT11 multifunctionality and biocompatibility, stimulating dental pulp stem cells proliferation and differentiation, indicates a potential as a bio-based dental material for regenerative Dentistry. Its potential as a bacterial collagenases inhibitor to reduce collagen degradation in root/dentinal caries can be further explored.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anacardium / Cárie Dentária Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anacardium / Cárie Dentária Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido