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Regulation of interleukin-6 and matrix metalloproteinases syntheses by bioflavonoids and photobiomodulation in human gingival fibroblasts.
Cardoso, Laís Medeiros; Pansani, Taisa Nogueira; de Souza Costa, Carlos Alberto; Basso, Fernanda Gonçalves.
Afiliação
  • Cardoso LM; Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP, 1680 Humaitá Street, Araraquara, São Paulo, 14801-903, Brazil.
  • Pansani TN; Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP, 1680 Humaitá Street, Araraquara, São Paulo, 14801-903, Brazil.
  • de Souza Costa CA; Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP, 1680 Humaitá Street, Araraquara, São Paulo, 14801-903, Brazil.
  • Basso FG; Department of Dentistry, Ribeirão Preto University (UNAERP), 2201 Costábile Romano Avenue, Ribeirão Preto, São Paulo, 14096-900, Brazil. fergbasso@gmail.com.
Lasers Med Sci ; 37(7): 2973-2987, 2022 Sep.
Article em En | MEDLINE | ID: mdl-35612681
This study aimed to evaluate the separately effects of bioflavonoids proanthocyanidins, from grape seed extract (GSE) and synthetic naringenin (NA), as well as photobiomodulation (PBM) by low-level laser therapy on interleukin (IL)-6 and matrix metalloproteinases (MMPs) syntheses by human gingival fibroblasts (HGF). For this purpose, a connective tissue exposure (ulceration) model of HGF, stimulated with tumor necrosis factor-alpha (TNF-α), was used. Initially, the highest non-cytotoxic and non-genotoxic concentrations of bioflavonoids were determined by cell viability and micronuclei formation assays. Then, HGF were exposed to different stimuli: culture medium (negative control), dimethyl sulfoxide (DMSO), TNF-α, NA, GSE, TNF-α + NA, TNF-α + GSE, PBM (3 J/cm2, 0.025 W, 780 nm), and TNF-α + PBM. Next, IL-6, MMP-2, and MMP-9 syntheses were assessed. The concentration of 10 µg/mL of bioflavonoids increased cell viability at 24 and 48 h and did not present cytotoxic or genotoxic effects on HGF after 24, 48, and 72 h of contact. This concentration was selected for the assessment of bioflavonoids potential in modulating inflammatory mediators. TNF-α exposure enhanced IL-6 (170%), MMP-2 (10%), and MMP-9 (20%) syntheses, while a decrease of MMP-2 by 55% after exposure to TNF-α + GSE and 20% after TNF-α + NA and TNF-α + PBM was observed. MMP-9 synthesis was decreased by 35% after TNF-α + NA, 20% after TNF-α + GSE, and 30% after PBM. IL-6 was down-regulated by GSE in the presence of TNF-α (80%). In conclusion, TNF-α up-regulated IL-6 and MMPs, while bioflavonoids and PBM down-regulated MMP-2 and MMP-9 syntheses; GSE also decreased IL-6 synthesis, demonstrating the individual promising potential of these therapies for ulceration management.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-6 / Metaloproteinase 2 da Matriz Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Lasers Med Sci Assunto da revista: BIOTECNOLOGIA / RADIOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Interleucina-6 / Metaloproteinase 2 da Matriz Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Lasers Med Sci Assunto da revista: BIOTECNOLOGIA / RADIOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido