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Use of Computerized Microtomography, Energy Dispersive Spectroscopy, Scanning Electron Microscopy, and Atomic Force Microscopy to Monitor Effects of Adding Calcium to Bleaching Gels.
Mendonça, L C; Rodrigues, Mla; Bicalho, A A; daSilva, G R; Quagliatto, P S; Soares, C J.
Afiliação
  • Mendonça LC; Ludmila Cavalcanti de Mendonça, DDS, MS, PhD student, Biomechanics Group, Department of Operative Dentistry and Dental Materials, professor at Technical School of Health, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Rodrigues M; Maria de Lara Araújo Rodrigues, DDS student, Biomechanics Group, Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Bicalho AA; Aline Arêdes Bicalho, DDS, MS, PhD, Biomechanics Group, Department of Operative Dentistry and Dental Materials, professor at Technical School of Health, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • daSilva GR; Gisele Rodrigues da Silva, DDS, MS, PhD, professor, Biomechanics Research Group, Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Quagliatto PS; Paulo Sérgio Quagliatto, DDS, MS, PhD, professor, Biomechanics Research Group, Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
  • Soares CJ; *Carlos José Soares, DDS, MS, PhD, professor and chairman, Biomechanics Research Group, Department of Operative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Uberlândia, MG, Brazil.
Oper Dent ; 47(3): E162-E173, 2022 May 01.
Article em En | MEDLINE | ID: mdl-35584324
OBJECTIVES: The aim of this study was to evaluate the mineral content, expressed by calcium (Ca) and phosphate (P), in dental enamel exposed to bleaching agents using micro-computed tomography (micro-CT), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and atomic force microscopy (AFM). METHODS: Sixty bovine dental enamel specimens were randomly divided into three groups (n=20): HP35ca (bleached using 35% hydrogen peroxide with Ca); HP35wca (bleached using 35% hydrogen peroxide without Ca); and control (without bleaching). Five specimens from each group were used for SEM and EDS analyses, 10 specimens were used for AFM analysis, and the remaining five specimens were used for micro-CT analysis. The pH of the gels was measured using a pH meter. The EDS and micro-CT data were analyzed using one-way ANOVA and Pearson's correlation test. The AFM data were analyzed using one-way ANOVA (α=0.05). RESULTS: The weight percentages of Ca and P obtained using EDS were similar between the bleached and control groups. Small, superficial changes were observed by SEM in the HP35wca group. The HP35ca group showed similar patterns to the control group. AFM results showed no significant changes in the enamel roughness in any of the tested groups. No significant difference in the volume or depth of structural enamel loss was found between gels with and without Ca. No mineral loss was observed in the dentin substrate. The EDS and micro-CT analysis data exhibited a high correlation (p<0.001). CONCLUSION: The addition of Ca to the bleaching gel had no beneficial effect on the bleached tooth enamel in terms of composition, mineral loss, and surface roughness. Micro-CT results exhibited a high correlation with the EDS results.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria por Raios X / Cálcio / Esmalte Dentário / Microtomografia por Raio-X / Clareadores Dentários / Microscopia Limite: Animals Idioma: En Revista: Oper Dent Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria por Raios X / Cálcio / Esmalte Dentário / Microtomografia por Raio-X / Clareadores Dentários / Microscopia Limite: Animals Idioma: En Revista: Oper Dent Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos