The adoption of resistance training (RT) programs has been shown to positively influence sports performance-related parameters. However, the chronic effects of maximal strength protocols on the performance of soccer players are not completely investigated. The aim of this study was to evaluate the effects of performing two repetition maximum (2RM) before a resistance training session on the physical performance of youth soccer players. Seventeen players (under-20 category) were allocated in one of the following groups: 2RM + resistance training group (2RM + RT, n = 8) and resistance training group (RTG, n = 9). Both groups performed the same RT protocol during experimental weeks. However, the 2RM + RT performed 1 set of 2RM for 4 exercises, previously to RT protocol. Pre and post intervention period, one repetition maximum of the back squat exercise (1RMSQUAT), sprint time (15m-sprint test), countermovement jump (CMJ), repeated sprint ability (RSABEST and RSAMEAN), and yo-yo intermittent recovery test level-2 (YYIRT2) were assessed. Total load lifted (TLL) during the experimental weeks was also collected. Significant increases in 1RMSQUAT (2RM + RT: +45.1%, d = 4.40; RTG: +32.3%, d = 1.84), 15m sprint (2RM + RT: -9.0%, d = 7.9; RTG: -8.8%%, d = 3.2), CMJ (2RM + RT: +2.3%, d = 0.17; RTG: +0.8%, d = 0.07), RSABEST (2RM + RT: -2.4%, d = 0.6; RTG: -2.3%, d = 1.04), RSAMEAN (2RM + RT: -2.9%, d = 1.33; RTG: -3.4%, d = 1.78), YYIRT2 (2RM + RT: +12.0%, d = 0.82; RTG: +12.1%, d = 0.63) (all p < 0,05) were observed for both groups on pre to post-intervention periods, with no significant difference between groups. Therefore, the 2RM + RT protocol did not promote additional increase on performance of young soccer players.