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Final irrigation protocols affect radicular dentin DMP1-CT expression, microhardness, and biochemical composition.
Retana-Lobo, Cristina; Ramírez-Mora, Tatiana; Murillo-Gómez, Fabian; Maria Guerreiro-Tanomaru, Juliane; Tanomaru-Filho, Mario; Reyes-Carmona, Jessie.
Afiliação
  • Retana-Lobo C; Department of Endodontics, Faculty of Dentistry, LICIFO, University of Costa Rica, SJO, Sabanilla, San José, 11502, Costa Rica.
  • Ramírez-Mora T; Department of Endodontics, Faculty of Dentistry, LICIFO, University of Costa Rica, SJO, Sabanilla, San José, 11502, Costa Rica.
  • Murillo-Gómez F; Dental Materials Research Laboratory, School of Dentistry, University of Costa Rica, SJO, Sabanilla, San José, 11502, Costa Rica.
  • Maria Guerreiro-Tanomaru J; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil.
  • Tanomaru-Filho M; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil.
  • Reyes-Carmona J; Department of Endodontics, Faculty of Dentistry, LICIFO, University of Costa Rica, SJO, Sabanilla, San José, 11502, Costa Rica. jessreyesc@hotmail.com.
Clin Oral Investig ; 26(8): 5491-5501, 2022 Aug.
Article em En | MEDLINE | ID: mdl-35499657
OBJECTIVE: To evaluate the effect of several final irrigation protocols on radicular dentin microhardness, biochemical composition, and DMP1-CT expression. MATERIALS AND METHODS: A total of 140 single-rooted human teeth were prepared with WaveOne Gold files and randomly distributed into 7 groups (n = 20) according to the final irrigation protocol: distilled water (DW); sodium hypochlorite-EDTA (NaOCl-EDTA); EDTA (EDTA); EDTA-NaOCl (EDTA-NaOCl); EDTA-chlorhexidine (EDTA-CHX); passive ultrasonic irrigation (PUI:NaOCl-EDTA); and PUI:NaOCl-EDTA-NaOCl. Dentin microhardness (n = 10) was evaluated in the root canal lumen using Vickers hardness tester. Immunohistochemical analysis (n = 5) was used to evaluate DMP1-CT expression. Dentin ultrastructure and biochemical composition were evaluated by using Raman and energy dispersive X-ray analysis (EDAX) (n = 5) with a scanning electron microscope (SEM). Analysis of variance (ANOVA) and Tukey test were performed (p˂0.05). RESULTS: Raman spectra of the organic content and DMP1-CT expression were lower at the lumen canal in EDTA-NaOCl, PUI:NaOCl-EDTA, and PUI:NaOCl-EDTA-NaOCl when compared to control (p < 0.05). EDAX showed reduced values for calcium and phosphorus in EDTA-NaOCl, PUI:NaOCl-EDTA, and PUI:NaOCl-EDTA-NaOCl. SEM microphotography's showed completely cleaned dentin, permeable tubules, and dentin erosion, mainly when PUI was used. NaOCl-EDTA presented significantly higher microhardness values than PUI:NaOCl-EDTA-NaOCl (p < 0.05). PUI:NaOCl-EDTA-NaOCl exhibited the lowest Vickers hardness values of all groups. CONCLUSION: The final irrigation protocols that used a final rinse with NaOCl and PUI showed a detrimental effect on radicular dentin DMP1-CT expression, biochemical composition, and microhardness. CLINICAL RELEVANCE: The adequate irrigation protocol could be advantageous to preserve the radicular dentin ultrastructure, promote adequate adhesion, and sustain favorable conditions for biomineralization and regeneration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Irrigantes do Canal Radicular / Cavidade Pulpar Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: Clin Oral Investig Assunto da revista: ODONTOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Irrigantes do Canal Radicular / Cavidade Pulpar Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: Clin Oral Investig Assunto da revista: ODONTOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Alemanha