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Antibacterial activity of biosynthesized silver nanoparticles (AgNps) against Pectobacterium carotovorum.
Pineda, Mayra Eleonora Beltrán; Lizarazo Forero, Luz Marina; Sierra Avila, Cesar Augusto.
Afiliação
  • Pineda MEB; Doctorado en Biotecnología UN, Grupo de Investigación en Macromoléculas UN, Grupo de Investigación Biología Ambiental UPTC, Grupo de investigación Gestión Ambiental- Universidad de Boyacá, Universidad Nacional de Colombia, Bogotá, Colombia.
  • Lizarazo Forero LM; Grupo de Investigación Biología Ambiental, Universidad Pedagógica y Tecnológica de Colombia, Tunja, Colombia. luz.lizarazo@uptc.edu.co.
  • Sierra Avila CA; Grupo de Investigación en Macromoléculas, Universidad Nacional de Colombia, Bogotá, Colombia.
Braz J Microbiol ; 53(3): 1175-1186, 2022 Sep.
Article em En | MEDLINE | ID: mdl-35486355
In a bioprospecting study of paramo soils cultivated with potato (Solanum tuberosum), 50 fungal isolates were obtained and evaluated for their nitrate reductase (NR) activity, given the role played by this enzyme in the biosynthesis of silver nanoparticles (AgNps). Five isolates strain with high NR activity belonging to Penicillium simplicissimum, Aspergillus niger, and Fusarium oxysporum species were selected, verifying the presence of the NR enzyme in their enzymatic extract. Later, these strains showed the ability to biosynthesize AgNps with distorted spherical shapes and sizes ranging from 15 to 45 nm. Subsequently, an antibiosis test was carried out by the agar diffusion method using glass fiber disks against the phytopathogenic agent Pectobacterium carotovorum, finding halos of inhibition of bacterial growth up to 15.3 mm using a 100 ppm solution of the AgNps obtained from F. oxysporum. These results contribute to generating the basis of a new alternative for the control of this phytopathogenic agent of potato, challenging to manage by traditional methods and of relevance at the post-harvest level.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solanum tuberosum / Nanopartículas Metálicas / Fusarium Idioma: En Revista: Braz J Microbiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solanum tuberosum / Nanopartículas Metálicas / Fusarium Idioma: En Revista: Braz J Microbiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Brasil