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IRF4 suppresses osteogenic differentiation of BM-MSCs by transcriptionally activating miR-636/DOCK9 axis.
Zhang, Xuepu; Zhang, Yue; Yang, Limin; Wu, Yuexin; Ma, Xiaohu; Tong, Gang; Ban, Zhaoliang; Zhao, Haosen.
Afiliação
  • Zhang X; Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, China.
  • Zhang Y; Dental Department, The Second Affiliated Hospital of Jinzhou Medical University, China.
  • Yang L; Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, China.
  • Wu Y; Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, China.
  • Ma X; Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, China.
  • Tong G; Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, China.
  • Ban Z; Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, China.
  • Zhao H; Orthopedics, The First Affiliated Hospital of Jinzhou Medical University, China. Electronic address: ZXP7536@163.com.
Clinics (Sao Paulo) ; 77: 100019, 2022.
Article em En | MEDLINE | ID: mdl-35397366
OBJECTIVES: Osteoblasts are derived from Bone Marrow-derived Mesenchymal Stem Cells (BM-MSCs), which play an indispensable role in bone formation. In this study, the authors aim to investigate the role of IRF4 in the osteogenic differentiation of BM-MSCs and its potential molecular mechanism. METHODS: The authors used lentivirus infection to overexpress IRF4 in BM-MSCs. The expression of IRF4 and osteogenesis-related genes were detected by qRT-PCR and western blot analysis. The osteogenic differentiation of BM-MSCs was evaluated by Alkaline Phosphatase (ALP) activity, Alizarin red staining, and Alkaline Phosphatase (ALP) staining. Chromatin Immunoprecipitation (ChIP), Dual-Luciferase reporter assay and RNA Immunoprecipitation Assay were applied to confirm the regulatory mechanism between IRF4, miR-636 and DOCK9. RESULTS: The authors found IRF4 was down-regulated during the osteogenic differentiation of BM-MSCs, and IRF4 overexpression could decrease the osteogenic differentiation of BM-MSCs by specifically promoting the reduction of Alkaline Phosphatase (ALP) activity and down-regulating osteogenic indicators, including OCN, OPN, Runx2 and CollA1. Mechanistically, IRF4 activated microRNA-636 (miR-636) expression via binding to its promoter region, and Dedicator of Cytokinesis 9 (DOCK9) was identified as the target of miR-636 in BM-MSCs. Moreover, the damage in the capacity of osteogenic differentiation of BM-MSCs induced by IRF4 overexpression could be rescued by miR-636 inhibition. CONCLUSIONS: In summary, this paper proposed that IRF4/miR-636/DOCK9 may be considered as targets for the treatment of osteoporosis (OP).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Troca do Nucleotídeo Guanina / MicroRNAs / Fatores Reguladores de Interferon / Células-Tronco Mesenquimais Tipo de estudo: Prognostic_studies Idioma: En Revista: Clinics (Sao Paulo) Assunto da revista: MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Troca do Nucleotídeo Guanina / MicroRNAs / Fatores Reguladores de Interferon / Células-Tronco Mesenquimais Tipo de estudo: Prognostic_studies Idioma: En Revista: Clinics (Sao Paulo) Assunto da revista: MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos