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Immunoassay platform with surface-enhanced resonance Raman scattering for detecting trace levels of SARS-CoV-2 spike protein.
Bistaffa, Maria J; Camacho, Sabrina A; Pazin, Wallance M; Constantino, Carlos J L; Oliveira, Osvaldo N; Aoki, Pedro H B.
Afiliação
  • Bistaffa MJ; São Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP, 19806-900, Brazil.
  • Camacho SA; São Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP, 19806-900, Brazil; IFSC, São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, 13566-590, Brazil. Electronic address: sabrina.alessio@gmail.com.
  • Pazin WM; IFSC, São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, 13566-590, Brazil; São Paulo State University (UNESP), School of Technology and Applied Sciences, 19060-900, Presidente Prudente, SP, Brazil.
  • Constantino CJL; São Paulo State University (UNESP), School of Technology and Applied Sciences, 19060-900, Presidente Prudente, SP, Brazil.
  • Oliveira ON; IFSC, São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, 13566-590, Brazil.
  • Aoki PHB; São Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP, 19806-900, Brazil.
Talanta ; 244: 123381, 2022 Jul 01.
Article em En | MEDLINE | ID: mdl-35364338
The early diagnosis of Coronavirus disease (COVID-19) requires either an accurate detection of genetic material or a sensitive detection of viral proteins. In this work, we designed an immunoassay platform for detecting trace levels of SARS-CoV-2 spike (S) protein. It is based on surface-enhanced resonance Raman scattering (SERRS) of methylene blue (MB) adsorbed onto spherical gold nanoparticles (AuNPs) and coated with a 6 nm silica shell. The latter shell in the SERRS nanoprobe prevented aggregation and permitted functionalization with SARS-CoV-2 antibodies. Specificity of the immunoassay was achieved by combining this functionalization with antibody immobilization on the cover slides that served as the platform support. Different concentrations of SARS-CoV-2 antigen could be distinguished and the lack of influence of interferents was confirmed by treating SERRS data with the multidimensional projection technique Sammon's mapping. With SERRS using a laser line at 633 nm, the lowest concentration of spike protein detected was 10 pg/mL, achieving a limit of detection (LOD) of 0.046 ng/mL (0.60 pM). This value is comparable to the lowest concentrations in the plasma of COVID-19 patients at the onset of symptoms, thus indicating that the SERRS immunoassay platform may be employed for early diagnosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Nanopartículas Metálicas / COVID-19 Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Nanopartículas Metálicas / COVID-19 Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda