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Methods to Evaluate Bacterial Motility and Its Role in Bacterial-Host Interactions.
Palma, Victoria; Gutiérrez, María Soledad; Vargas, Orlando; Parthasarathy, Raghuveer; Navarrete, Paola.
Afiliação
  • Palma V; Laboratory of Microbiology and Probiotics, Institute of Nutrition and Food Technology (INTA), University of Chile, El Líbano 5524, Santiago 7830490, Chile.
  • Gutiérrez MS; Laboratory of Microbiology and Probiotics, Institute of Nutrition and Food Technology (INTA), University of Chile, El Líbano 5524, Santiago 7830490, Chile.
  • Vargas O; Millennium Science Initiative Program, Milenium Nucleus in the Biology of the Intestinal Microbiota, National Agency for Research and Development (ANID), Moneda 1375, Santiago 8200000, Chile.
  • Parthasarathy R; Laboratory of Microbiology and Probiotics, Institute of Nutrition and Food Technology (INTA), University of Chile, El Líbano 5524, Santiago 7830490, Chile.
  • Navarrete P; Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.
Microorganisms ; 10(3)2022 Mar 04.
Article em En | MEDLINE | ID: mdl-35336138
Bacterial motility is a widespread characteristic that can provide several advantages for the cell, allowing it to move towards more favorable conditions and enabling host-associated processes such as colonization. There are different bacterial motility types, and their expression is highly regulated by the environmental conditions. Because of this, methods for studying motility under realistic experimental conditions are required. A wide variety of approaches have been developed to study bacterial motility. Here, we present the most common techniques and recent advances and discuss their strengths as well as their limitations. We classify them as macroscopic or microscopic and highlight the advantages of three-dimensional imaging in microscopic approaches. Lastly, we discuss methods suited for studying motility in bacterial-host interactions, including the use of the zebrafish model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Microorganisms Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Chile País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Microorganisms Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Chile País de publicação: Suíça