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Effects of Magnetite Nanoparticles and Static Magnetic Field on Neural Differentiation of Pluripotent Stem Cells.
Semeano, Ana T; Tofoli, Fabiano A; Corrêa-Velloso, Juliana C; de Jesus Santos, Ana P; Oliveira-Giacomelli, Ágatha; Cardoso, Rafaela R; Pessoa, Mateus A; da Rocha, Edroaldo Lummertz; Ribeiro, Gustavo; Ferrari, Merari F R; Pereira, Lygia V; Teng, Yang D; Petri, Denise F S; Ulrich, Henning.
Afiliação
  • Semeano AT; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748. Sala 964 Bloco 9 Superior, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.
  • Tofoli FA; Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748. Sala 307 Bloco 3 Inferior, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.
  • Corrêa-Velloso JC; Department of Pharmaceutical Sciences, School of Pharmacy, Bouvé College of Health Sciences, Northeastern University, Boston, MA, 02115, USA.
  • de Jesus Santos AP; Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Oliveira-Giacomelli Á; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748. Sala 964 Bloco 9 Superior, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.
  • Cardoso RR; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748. Sala 964 Bloco 9 Superior, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.
  • Pessoa MA; Department of Biochemistry, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes, 748. Sala 964 Bloco 9 Superior, Cidade Universitária, São Paulo, SP, 05508-000, Brazil.
  • da Rocha EL; Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Ribeiro G; Department of Microbiology, Immunology and Parasitology at Federal University of Santa Catarina, Florianópolis, Brazil.
  • Ferrari MFR; Department of Microbiology, Immunology and Parasitology at Federal University of Santa Catarina, Florianópolis, Brazil.
  • Pereira LV; Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Teng YD; Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Petri DFS; Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Ulrich H; Laboratory of SCI, Stem Cell and Recovery Neurobiology Research, Department of Physical Medicine & Rehabilitation, Harvard Medical School, Spaulding Rehabilitation Hospital Network, and Mass General Brigham, Boston, MA, USA.
Stem Cell Rev Rep ; 18(4): 1337-1354, 2022 04.
Article em En | MEDLINE | ID: mdl-35325357
Neurodevelopmental processes of pluripotent cells, such as proliferation and differentiation, are influenced by external natural forces. Despite the presence of biogenic magnetite nanoparticles in the central nervous system and constant exposure to the Earth's magnetic fields and other sources, there is scant knowledge regarding the role of electromagnetic stimuli in neurogenesis. Moreover, emerging applications of electrical and magnetic stimulation to treat neurological disorders emphasize the relevance of understanding the impact and mechanisms behind these stimuli. Here, the effects of magnetic nanoparticles (MNPs) in polymeric coatings and the static external magnetic field (EMF) were investigated on neural induction of murine embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs). The results show that the presence of 0.5% MNPs in collagen-based coatings facilitates the migration and neuronal maturation of mESCs and hiPSCs in vitro. Furthermore, the application of 0.4 Tesla EMF perpendicularly to the cell culture plane, discernibly stimulates proliferation and guide fate decisions of the pluripotent stem cells, depending on the origin of stem cells and their developmental stage. Mechanistic analysis reveals that modulation of ionic homeostasis and the expression of proteins involved in cytostructural, liposomal and cell cycle checkpoint functions provide a principal underpinning for the impact of electromagnetic stimuli on neural lineage specification and proliferation. These findings not only explore the potential of the magnetic stimuli as neural differentiation and function modulator but also highlight the risks that immoderate magnetic stimulation may affect more susceptible neurons, such as dopaminergic neurons.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Células-Tronco Pluripotentes Induzidas / Nanopartículas de Magnetita Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Stem Cell Rev Rep Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Células-Tronco Pluripotentes Induzidas / Nanopartículas de Magnetita Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Stem Cell Rev Rep Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos