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The use of insulin-transferrin-selenium (ITS), and folic acid on individual in vitro embryo culture systems in cattle.
Dos Santos Mendonça-Soares, Anelise; Guimarães, Ana Luiza Silva; Fidelis, Andrei Antonioni Guedes; Franco, Maurício Machaim; Dode, Margot Alves Nunes.
Afiliação
  • Dos Santos Mendonça-Soares A; Federal Institute of Education, Science and Technology of Triângulo Mineiro, Uberaba Parque Tecnológico Campus, Uberaba, Brazil.
  • Guimarães ALS; Lutheran University Center of Palmas - CEULP ULBRA, Palmas, Brazil.
  • Fidelis AAG; Veterinary Department, University Center of Brasília-UniCeub, Brasília, Brazil.
  • Franco MM; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, Brasília, Brazil; Institute of Biotechnology, Federal University of Uberlândia, Uberlândia, Brazil; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia, Brazil.
  • Dode MAN; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Parque Estação Biológica, Brasília, Brazil; School of Agriculture and Veterinary, University of Brasília, Brasília, Brazil. Electronic address: margot.dode@embrapa.br.
Theriogenology ; 184: 153-161, 2022 May.
Article em En | MEDLINE | ID: mdl-35316734
Individual embryo culture is the only strategy that allows the tracking of embryos throughout the culture period. However, this procedure leads to lower embryo development. This study aimed to evaluate different alternatives to improve embryo development in a single in vitro production system. First, embryo production was compared between individual cultures on a 20 µL droplet and Cell-Tak® system. Then, various concentrations of folic acid were tested for use in combination with insulin-transferrin-selenium (ITS). To determine the concentration, embryos were analyzed not only by development but also by their methylation status. Finally, the supplementation of individual culture media with ITS and/or folic acid was evaluated. The results showed that embryos cultured in the Cell-Tak® system presented lower blastocyst rates than the microdroplets system. When the concentration of folic acid was tested, 20 µM and 500 µM presented a higher level of insulin-like growth factor (IGF2) DNA methylation pattern compared to control, suggesting that in vitro conditions alter DNA methylation pattern in that region and folic acid reestablishes the pattern. However, when it was used in an individual culture system, folic acid did not improve embryo development. Conversely, ITS which is composed of three important components, proved to be an alternative to individual embryo culture, improving embryo rates, showing similar rates to grouped culture embryos. Since Folic Acid change epigenetic profile, additional studies are needed to evaluate its use in IVP culture systems.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Selênio / Técnicas de Cultura Embrionária Limite: Animals Idioma: En Revista: Theriogenology Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Selênio / Técnicas de Cultura Embrionária Limite: Animals Idioma: En Revista: Theriogenology Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos