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Usefulness of temperate-adapted maize lines developed by doubled haploid and single-seed descent methods.
Santos, Iara Gonçalves Dos; Verzegnazzi, Anderson Luiz; Edwards, Jode; Frei, Ursula K; Boerman, Nicholas; Tonello Zuffo, Leandro; Pires, Luiz P M; de La Fuente, Gerald; Lübberstedt, Thomas.
Afiliação
  • Santos IGD; Department of General Biology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil. iara.santos@ufv.br.
  • Verzegnazzi AL; Department of Agronomy, Iowa State University, Ames, IA, USA.
  • Edwards J; USDA-ARS, Corn Insects and Crop Genetics Research Unit, Ames, IA, USA.
  • Frei UK; Department of Agronomy, Iowa State University, Ames, IA, USA.
  • Boerman N; USDA-ARS, Southern Plains Range Research Station, Woodward, OK, USA.
  • Tonello Zuffo L; Department of Plant Sciences, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
  • Pires LPM; KWS, Londrina, Parana, Brazil.
  • de La Fuente G; SESACO, Austin, TX, USA.
  • Lübberstedt T; Department of Agronomy, Iowa State University, Ames, IA, USA.
Theor Appl Genet ; 135(6): 1829-1841, 2022 Jun.
Article em En | MEDLINE | ID: mdl-35305125
KEY MESSAGE: Spontaneous haploid genome doubling is not associated with undesirable linkage drag effects. The presence of spontaneous doubling genes allows maximum exploitation of variability from the temperate-adapted BS39 population Tropical non-elite maize (Zea mays L.) germplasm, such as BS39, provides a unique opportunity for broadening the genetic base of U.S. Corn Belt germplasm. In vivo doubled haploid (DH) technology has been used to efficiently exploit non-elite germplasm. It can help to purge deleterious recessive alleles. The objectives of this study were to determine the usefulness of BS39-derived inbred lines using both SSD and DH methods, to determine the impact of spontaneous as compared with artificial haploid genome doubling on genetic variance among BS39-derived DH lines, and to identify SNP markers associated with agronomic traits among BS39 inbreds monitored at testcross level. We developed two sets of inbred lines directly from BS39 by DH and SSD methods, named BS39_DH and BS39_SSD. Additionally, two sets were derived from a cross between BS39 and A427 (SHGD donor) by DH and SSD methods, named BS39 × A427_DH and BS39 × A427_SSD, respectively. Grain yield, moisture, plant height, ear height, stalk lodging, and root lodging were measured to estimate genetic parameters. For genome-wide association analysis, inbred lines were genotyped using genotype-by-sequencing and Diversity Array Technology Sequencing (DArTSeq). Some BS39-derived inbred lines performed better than elite germplasm inbreds and all sets showed significant genetic variance. The presence of spontaneous haploid genome doubling genes did not affect performance of inbred lines. Five SNPs were significant and three of them located within genes related to plant development or abiotic stresses. These results demonstrate the potential of BS39 to add novel alleles to temperate elite germplasm.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zea mays / Estudo de Associação Genômica Ampla Idioma: En Revista: Theor Appl Genet Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zea mays / Estudo de Associação Genômica Ampla Idioma: En Revista: Theor Appl Genet Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha