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Comparative venomic profiles of three spiders of the genus Phoneutria.
Fernandes, Frederico Francisco; Moraes, Juliana Rodrigues; Dos Santos, Jaqueline Leal; Soares, Thiago Geraldo; Gouveia, Vitor José Pinto; Matavel, Alessandra C S; Borges, William de Castro; Cordeiro, Marta do Nascimento; Figueiredo, Suely Gomes; Borges, Márcia Helena.
Afiliação
  • Fernandes FF; Laboratory of Proteomics and Arachnids, Research and Development Board, Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • Moraes JR; Laboratory of Enzymology and Proteomics, Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto, MG, Brazil.
  • Dos Santos JL; Laboratory of Proteomics and Arachnids, Research and Development Board, Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • Soares TG; Laboratory of Proteomics and Arachnids, Research and Development Board, Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • Gouveia VJP; Laboratory of Proteomics and Arachnids, Research and Development Board, Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • Matavel ACS; Laboratory of Proteomics and Arachnids, Research and Development Board, Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • Borges WC; Laboratory of Biotechnology and Health, Research and Development Board, Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • Cordeiro MDN; Laboratory of Enzymology and Proteomics, Department of Biological Sciences, Federal University of Ouro Preto, Ouro Preto, MG, Brazil.
  • Figueiredo SG; Laboratory of Proteomics and Arachnids, Research and Development Board, Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
  • Borges MH; Laboratory of Protein Chemistry, Department of Physiological Sciences, Federal University of Espírito Santo, Vitória, ES, Brazil.
Article em En | MEDLINE | ID: mdl-35283937
Background: Spider venoms induce different physio-pharmacological effects by binding with high affinity on molecular targets, therefore being of biotechnological interest. Some of these toxins, acting on different types of ion channels, have been identified in the venom of spiders of the genus Phoneutria, mainly from P. nigriventer. In spite of the pharmaceutical potential demonstrated by P. nigriventer toxins, there is limited information on molecules from venoms of the same genus, as their toxins remain poorly characterized. Understanding this diversity and clarifying the differences in the mechanisms of action of spider toxins is of great importance for establishing their true biotechnological potential. This prompted us to compare three different venoms of the Phoneutria genus: P. nigriventer (Pn-V), P. eickstedtae (Pe-V) and P. pertyi (Pp-V). Methods: Biochemical and functional comparison of the venoms were carried out by SDS-PAGE, HPLC, mass spectrometry, enzymatic activities and electrophysiological assays (whole-cell patch clamp). Results: The employed approach revealed that all three venoms had an overall similarity in their components, with only minor differences. The presence of a high number of similar proteins was evident, particularly toxins in the mass range of ~6.0 kDa. Hyaluronidase and proteolytic activities were detected in all venoms, in addition to isoforms of the toxins Tx1 and Tx2-6. All Tx1 isoforms blocked Nav1.6 ion currents, with slight differences. Conclusion: Our findings showed that Pn-V, Pe-V and Pp-V are highly similar concerning protein composition and enzymatic activities, containing isoforms of the same toxins sharing high sequence homology, with minor modifications. However, these structural and functional variations are very important for venom diversity. In addition, our findings will contribute to the comprehension of the molecular diversity of the venoms of the other species from Phoneutria genus, exposing their biotechnological potential as a source for searching for new active molecules.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Venom Anim Toxins Incl Trop Dis Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: J Venom Anim Toxins Incl Trop Dis Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil