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Proteomic Analysis of Sporothrix schenckii Exposed to Oxidative Stress Induced by Hydrogen Peroxide.
Saucedo-Campa, Dulce O; Martínez-Rocha, Ana L; Ríos-Castro, Emmanuel; Alba-Fierro, Carlos A; Escobedo-Bretado, Miguel A; Cuéllar-Cruz, Mayra; Ruiz-Baca, Estela.
Afiliação
  • Saucedo-Campa DO; Facultad de Ciencias Químicas (Unidad Durango), Universidad Juárez del Estado de Durango, Av. Veterinaria S/N, Durango 34120, Mexico.
  • Martínez-Rocha AL; Facultad de Ciencias Químicas (Unidad Durango), Universidad Juárez del Estado de Durango, Av. Veterinaria S/N, Durango 34120, Mexico.
  • Ríos-Castro E; Centro de Investigación y de Estudios Avanzados del I.P.N., Unidad de Genómica, Proteómica y Metabolómica, LaNSE, Ciudad de Mexico 07360, Mexico.
  • Alba-Fierro CA; Facultad de Ciencias Químicas (Unidad Durango), Universidad Juárez del Estado de Durango, Av. Veterinaria S/N, Durango 34120, Mexico.
  • Escobedo-Bretado MA; Facultad de Ciencias Químicas (Unidad Durango), Universidad Juárez del Estado de Durango, Av. Veterinaria S/N, Durango 34120, Mexico.
  • Cuéllar-Cruz M; Departamento de Biología, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta S/N, Guanajuato 36050, Mexico.
  • Ruiz-Baca E; Facultad de Ciencias Químicas (Unidad Durango), Universidad Juárez del Estado de Durango, Av. Veterinaria S/N, Durango 34120, Mexico.
Pathogens ; 11(2)2022 Feb 10.
Article em En | MEDLINE | ID: mdl-35215174
Sporothrix schenckii modulates the expression of its cell wall proteins (CWPs) in response to reactive oxygen species (ROS) generated by the phagocytic cells of the human host, which allows it to evade and escape the immune system. In this study, we performed a comparative proteomic analysis of the CW of S. schenckii after exposure and nonexposure to H2O2. Several CWPs involved in CW remodeling and fungal pathogenesis that modulated their expression in response to this oxidizing agent were identified, as were a number of antioxidant enzymes and atypical CWPs, called moonlighting proteins, such as the Hsp70-5, lipase 1 (Lip1), enolase (Eno), and pyruvate kinase (Pk). Moreover, RT-qPCR assays demonstrated that the transcription of genes HSP70-5, LIP1, ENO, and PK is regulated in response to the oxidant. The results indicated that S. schenckii differentially expressed CWPs to confer protection against ROS upon this fungus. Furthermore, among these proteins, antioxidant enzymes and interestingly, moonlighting-like CWPs play a role in protecting the fungus from oxidative stress (OS), allowing it to infect human host cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pathogens Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pathogens Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México País de publicação: Suíça