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Main lymphocyte subpopulations in cerebrospinal fluid and peripheral blood in HIV-1 subtypes C and B.
de Almeida, Sergio M; Beltrame, Miriam Perlingeiro; Tang, Bin; Rotta, Indianara; Schluga, Yara; Justus, Julie Lilian P; da Rocha, Maria Tadeu; Abramson, Ian; Vaida, Florin; Schrier, Rachel; Ellis, Ronald J.
Afiliação
  • de Almeida SM; Laboratório de Virologia, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil. sergio.ma@ufpr.br.
  • Beltrame MP; Laboratório de Citometria de Fluxo, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Tang B; HNRC- HIV Neurobehavioral Research Center, UCSD, San Diego, CA, USA.
  • Rotta I; Laboratório de Virologia, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Schluga Y; Laboratório de Citometria de Fluxo, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Justus JLP; Laboratório de Citometria de Fluxo, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • da Rocha MT; Laboratório de Citometria de Fluxo, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Abramson I; HNRC- HIV Neurobehavioral Research Center, UCSD, San Diego, CA, USA.
  • Vaida F; HNRC- HIV Neurobehavioral Research Center, UCSD, San Diego, CA, USA.
  • Schrier R; HNRC- HIV Neurobehavioral Research Center, UCSD, San Diego, CA, USA.
  • Ellis RJ; HNRC- HIV Neurobehavioral Research Center, UCSD, San Diego, CA, USA.
J Neurovirol ; 28(2): 291-304, 2022 04.
Article em En | MEDLINE | ID: mdl-35190973
HIV-1 subtype C (HIV-1C) shows reduced Tat protein chemoattractant activity compared with HIV-1B. The impact of HIV-1C Tat on the chemotaxis of the main lymphocyte subpopulations in the cerebrospinal fluid (CSF) and the peripheral blood (PB) is unclear. We hypothesized that there would be a lower frequency of specific lymphocyte subpopulations CD3+ or CD19+ in CSF in HIV-1C than in HIV-1B. The objectives were to detect the differences in the proportions of main lymphocyte subpopulations in CSF and PB, between people with HIV (PWH) and HIV-1-uninfected volunteers (PWoH) and in HIV-1B and HIV-1C. Lymphocyte immunophenotyping was studied in CSF and paired PB samples of PWH (n = 22) and PWoH (n = 14). Lymphocytes were analyzed within the CD45+ gated region. The proportions of CSF CD3+CD4+, CD3+CD8+, and CD3-CD19+ lymphocytes in CSF were comparable in HIV-1B and C. There was an increase in the proportion of CD3+CD8+ cells and a decrease in CD3+CD4+ T cells (ps = 0.016) in the CSF samples of the PWH compared with the PWoH group. In the PWH group, both CD3+CD4+ and CD3+CD8+ lymphocytes were significantly higher in the CSF than in the PB (p = 0.047 and 0.005). The proportion of CD3+CD4+ was lower and that of CD3+CD8+ was higher in the CSF samples of the aviremic group than that of HIV-negative control (p = 0.0008 and < 0.0001, respectively). HIV-1C Tat substitution (C30S) did not interfere with the CNS migration of the main lymphocyte subpopulations. This is the first study to evaluate these lymphocytes in CSF and PB of HIV-1C compared with HIV-1B.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por HIV / HIV-1 Limite: Humans Idioma: En Revista: J Neurovirol Assunto da revista: NEUROLOGIA / VIROLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por HIV / HIV-1 Limite: Humans Idioma: En Revista: J Neurovirol Assunto da revista: NEUROLOGIA / VIROLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos