LncRNA ABHD11-AS1 promotes tumor progression in papillary thyroid carcinoma by regulating EPS15L1/EGFR signaling pathway.

Lu, H; Zhu, C; Chen, Y; Ruan, Y; Fan, L; Chen, Q; Wei, Q

Lu, H; Zhu, C; Chen, Y; Ruan, Y; Fan, L; Chen, Q; Wei, Q.

Afiliação

Lu H; Department of Pathology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.
Zhu C; Department of Pathology, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, 318000, China.
Chen Y; Precision Medicine Center, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, 318000, People's Republic of China.
Ruan Y; Department of Pathology, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, 318000, China.
Fan L; Precision Medicine Center, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, 318000, People's Republic of China.
Chen Q; Department of Pathology, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, 318000, China.
Wei Q; Precision Medicine Center, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, 318000, People's Republic of China. chenq5075@tzzxyy.com.

Clin Transl Oncol ; 24(6): 1124-1133, 2022 Jun.

Article em En | MEDLINE | ID: mdl-35098448

RESUMO

OBJECTIVES: lncRNA ABHD11 antisense RNA 1 (ABHD11-AS1) acts as an oncogene involved in papillary thyroid carcinoma (PTC) occurrence and progression. ABHD11-AS1 exerts biologic functions by some miRNAs and proteins to regulate multiple targets. Identification of novel mechanism of ABHD11-AS1 could be helpful in therapeutic targeting for PTC treatment. METHODS: Differentially expressed lncRNAs were selected from TCGA database. qRT-PCR analysis was applied to examine the expression of ABHD11-AS1 in PTC cell lines and tissues. The relationship of ABHD11-AS1 expression and clinicopathological features was analyzed by Kaplan-Meier analysis. Two PTC cell lines (TPC-1 and KTC-1) were transfected with pcDNA 3.1, pcDNA3.1-ABHD11-AS1, si-NC and si-ABHD11-AS1, respectively, to verify the ABHD11-AS1 oncogene-regulating capacity to promote tumor progression. The cell metastasis and proliferation had been evaluated both in vitro and in vivo. RESULTS: High expression of ABHD11-AS1 was found in PTC tissues (P < 0.01), which was significantly correlated with lymph node metastasis (P < 0.05). ABHD11-AS1 overexpression noticeably promoted cell proliferation, migration, and invasion capabilities, which were obviously decreased upon ABHD11-AS1 knockdown. ABHD11-AS1 positively regulated EGFR/EPS15L1 pathway, as EGFR, EPS15L1, STAT3, and p-STAT3 were activated. CONCLUSION: ABHD11-AS1 promotes tumor progression in PTC by regulating EPS15L1/EGFR pathway.

Assuntos

MicroRNAs; RNA Longo não Codificante; Neoplasias da Glândula Tireoide; Linhagem Celular Tumoral; Movimento Celular/genética; Proliferação de Células/genética; Receptores ErbB/genética; Receptores ErbB/metabolismo; Regulação Neoplásica da Expressão Gênica; Humanos; MicroRNAs/genética; RNA Antissenso/genética; RNA Longo não Codificante/genética; RNA Longo não Codificante/metabolismo; Serina Proteases/genética; Serina Proteases/metabolismo; Transdução de Sinais/genética; Câncer Papilífero da Tireoide/genética; Neoplasias da Glândula Tireoide/patologia

Palavras-chave

ABHD11-AS1; EGFR; EPS15L1; Papillary thyroid carcinoma; STAT3

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Glândula Tireoide / MicroRNAs / RNA Longo não Codificante Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Clin Transl Oncol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Itália

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