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Genome-wide analysis reveals a rhamnolipid-dependent modulation of flagellar genes in Pseudomonas aeruginosa PAO1.
Castro, Michele R; Dias, Graciela M; Salles, Tiago S; Cabral, Nubia M; Mariano, Danielly C O; Oliveira, Hadassa L; Abdelhay, Eliana S F W; Binato, Renata; Neves, Bianca C.
Afiliação
  • Castro MR; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
  • Dias GM; Department of Biology, Federal Institute of Rio de Janeiro (IFRJ), Rio de Janeiro, Brazil.
  • Salles TS; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
  • Cabral NM; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
  • Mariano DCO; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
  • Oliveira HL; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
  • Abdelhay ESFW; Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos, 149, Lab A537, Rio de Janeiro, RJ, 21941-919, Brazil.
  • Binato R; National Cancer Institute (INCA), Rio de Janeiro, Brazil.
  • Neves BC; National Cancer Institute (INCA), Rio de Janeiro, Brazil.
Curr Genet ; 68(2): 289-304, 2022 Apr.
Article em En | MEDLINE | ID: mdl-35094149
Pseudomonas aeruginosa is an opportunistic pathogen and an important model organism for the study of bacterial group behaviors, including cell motility and biofilm formation. Rhamnolipids play a pivotal role in biofilm formation and motility phenotypes in P. aeruginosa, possibly acting as wetting agents and mediating chemotactic stimuli. However, no biochemical mechanism or gene regulatory network has been investigated in regard to rhamnolipids' modulation of those group behaviors. Using DNA microarrays, we investigated the transcriptomic profiles in the stationary phase of growth of wild-type P. aeruginosa PAO1 and a rhlA-mutant strain, unable to produce rhamnolipids. A total of 134 genes were differentially expressed, comprising different functional categories, indicating a significant physiological difference between the rhamnolipid-producing and -non-producing strains. Interestingly, several flagellar genes are repressed in the mutant strain, which directly relates to the inability of the rhlA-minus strain to develop a swarming-motility phenotype. Supplementation with exogenous rhamnolipids has partially restored flagellar gene expression in the mutant strain. Our results show significant evidence that rhamnolipids, the major biosynthetic products of rhlABC pathway, seem to modulate gene expression in P. aeruginosa.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Glicolipídeos Idioma: En Revista: Curr Genet Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Glicolipídeos Idioma: En Revista: Curr Genet Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos