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Punicalin and ellagic acid from pomegranate peel extract facilitate apoptotic behaviour in the Hela cell line.
Gonzalez-Castillo, Marily; Jesus Loera, Maria de; Ascacio-Valdes, Juan; Rodríguez-Herrera, Raul; Zugasti-Cruz, Alejandro; Salinas-Santander, Mauricio; Cepeda-Nieto, Ana Cecilia; Vera-Reyes, Ileana; Ángel-Martínez, Mayela Del; Morlett-Chavez, Antonio.
Afiliação
  • Gonzalez-Castillo M; Molecular Biology Laboratory, Food Research Department, Chemistry School, Autonomous University of Coahuila (UAdeC), Coahuila, Mexico.
  • Jesus Loera M; Histology Laboratory, Histology Department, Medicine School, Autonomous University of Nuevo Leon. Nuevo Leon, Mexico.
  • Ascacio-Valdes J; Molecular Biology Laboratory, Food Research Department, Chemistry School, Autonomous University of Coahuila (UAdeC), Coahuila, Mexico.
  • Rodríguez-Herrera R; Molecular Biology Laboratory, Food Research Department, Chemistry School, Autonomous University of Coahuila (UAdeC), Coahuila, Mexico.
  • Zugasti-Cruz A; Toxicology Laboratory, Food Research Department, Chemistry School, Autonomous University of Coahuila (UAdeC), Coahuila, Mexico.
  • Salinas-Santander M; Molecular Biology Laboratory, Health Research Department, Medicine School at Saltillo, Autonomous University of Coahuila, Coahuila, Mexico.
  • Cepeda-Nieto AC; Molecular Biology Laboratory, Health Research Department, Medicine School at Saltillo, Autonomous University of Coahuila, Coahuila, Mexico.
  • Vera-Reyes I; Proteomics Laboratory, Agricultural Plant Science and Biotechnology, Centro de Investigación en Química Aplicada. Coahuila, Mexico.
  • Ángel-Martínez MD; Molecular Biology Laboratory, Health Research Department, Medicine School at Saltillo, Autonomous University of Coahuila, Coahuila, Mexico.
  • Morlett-Chavez A; Molecular Biology Laboratory, Food Research Department, Chemistry School, Autonomous University of Coahuila (UAdeC), Coahuila, Mexico/Clinical Analysis and Molecular Diagnosis Laboratory, Autonomous University of Coahuila. Coahuila, Mexico/Molecular Biology Laboratory, Health Research Department, Me
Pak J Pharm Sci ; 34(6): 2181-2189, 2021 Nov.
Article em En | MEDLINE | ID: mdl-35034879
Polyphenols may be an effective therapy for both the prevention and treatment of cancer. Previous studies have found that these compounds may inactive Hela cells, which may even be converted into a normal cells post-treatment. The present study extracted phenolic compounds from pomegranate peel, with the polyphenols then purified using different solvents and identified by means of high-performance liquid chromatography-tandem mass spectrometry (HPLC/MS). Once the phenolic compounds had been purified, we evaluated their cytotoxic effects on both the Hela and NIH-3T3 cell lines, on which an apoptosis assay was also carried out. Additionally, apoptosis assay was carried out on Hela and NIH-3T3. Lastly, the proteome profile was analysed via two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC/MS/MS). We isolated and then purified punicalagin and ellagic acid (EA) from pomegranate peel, with both compounds likely to have a cytotoxic effect on Hela and NIH-3T3. However, this effect depends on both concentration and exposure time. Results obtained using a Cayman commercial assay kit suggests that punicalin and EA regulate the apoptosis on the Hela and NIH-3T3 cell lines. Finally, we observed that polyphenols compounds regulate the expression of proteins related to apoptosis. In conclusion, punicalin and EA have a cytotoxic effect on Hela and, furthermore, reactive the apoptotic pathway in this cell.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Neoplasias do Colo do Útero / Apoptose / Taninos Hidrolisáveis / Ácido Elágico / Punica granatum / Antineoplásicos Tipo de estudo: Prognostic_studies Limite: Animals / Female / Humans Idioma: En Revista: Pak J Pharm Sci Assunto da revista: FARMACIA / FARMACOLOGIA / QUIMICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Paquistão
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Neoplasias do Colo do Útero / Apoptose / Taninos Hidrolisáveis / Ácido Elágico / Punica granatum / Antineoplásicos Tipo de estudo: Prognostic_studies Limite: Animals / Female / Humans Idioma: En Revista: Pak J Pharm Sci Assunto da revista: FARMACIA / FARMACOLOGIA / QUIMICA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Paquistão