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Development and Validation of a Novel ELISA for the Specific Detection of Antibodies against Mycobacterium avium Subspecies paratuberculosis Based on a Chimeric Polyprotein.
Moyano, Roberto Damián; Romero, Magali Andrea; Colombatti Olivieri, María Alejandra; Alvarado Pinedo, María Fiorella; Traveria, Gabriel Eduardo; Romano, María Isabel; Alonso, María Natalia.
Afiliação
  • Moyano RD; Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Agrobiotecnología y Biología Molecular (IABiMo), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Nicolás Repetto y Los Reseros, P.O. Box 1686, Hurlingham, Buenos Aires, Argentina.
  • Romero MA; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290 (C1425FQB), CABA, Argentina.
  • Colombatti Olivieri MA; Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Agrobiotecnología y Biología Molecular (IABiMo), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Nicolás Repetto y Los Reseros, P.O. Box 1686, Hurlingham, Buenos Aires, Argentina.
  • Alvarado Pinedo MF; Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE) de la Facultad de Ciencias Veterinarias, Universidad de La Plata, Alvear 803, P.O. Box 7130, Chascomus, Buenos Aires, Argentina.
  • Traveria GE; Centro de Diagnóstico e Investigaciones Veterinarias (CEDIVE) de la Facultad de Ciencias Veterinarias, Universidad de La Plata, Alvear 803, P.O. Box 7130, Chascomus, Buenos Aires, Argentina.
  • Romano MI; Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Agrobiotecnología y Biología Molecular (IABiMo), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Nicolás Repetto y Los Reseros, P.O. Box 1686, Hurlingham, Buenos Aires, Argentina.
  • Alonso MN; Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Agrobiotecnología y Biología Molecular (IABiMo), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Nicolás Repetto y Los Reseros, P.O. Box 1686, Hurlingham, Buenos Aires, Argentina.
Vet Med Int ; 2021: 7336848, 2021.
Article em En | MEDLINE | ID: mdl-35003619
Bovine paratuberculosis (PTB) is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The optimization of detection tests specific for MAP is crucial to improve PTB control. In this work, we aimed to develop and validate a diagnostic tool based on an ELISA to specifically detect anti-MAP antibodies from bovine serum samples. For that purpose, we designed a recombinant polyprotein containing four specific antigens from MAP and optimized the ELISA. The validation consisted of the assessment of 10 sera from PTB-infected and healthy bovines with different OD values. The diagnostic performance of the polyprotein-ELISA was evaluated by testing 130 bovine serum samples (47 healthy, 48 MAP-infected, and 35 M. bovis-infected bovines). The ELISA using the polyprotein yielded an area under the ROC curve (AUC) of 0.9912 (95% CI, 0.9758-1.007; P < 0.0001). Moreover, for this ELISA, the cut-off selected from the ROC curve based on the point with a sensitivity of 95.56% (95% CI, 0.8485-0.9946) and specificity of 97.92 (95% CI, 0.8893-0.9995) was 0.3328. Similar results were obtained with an ELISA using the commercial Paratuberculosis Protoplasmatic Antigen (PPA). However, the ELISA with the polyprotein antigen showed a better performance against sera from animals infected with Mycobacterium bovis compared to the ELISA with PPA: lower cross-reactivity (2.85% versus 25.71%). These results demonstrate a very low cross-reactivity of the polyprotein with antibodies present in serum samples from animals infected with M. bovis. The designed polyprotein and the validated ELISA could be very useful for the specific identification of MAP-infected animals in herds.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Vet Med Int Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Vet Med Int Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos