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Patulin inhibits LPS-induced nitric oxide production by suppressing MAPKs signaling pathway.
Sales-Neto, José Marreiro de; da Silva, Juliane Santos de França; Carvalho, Deyse Cristina Madruga; Lima, Éssia de Almeida; Cavalcante-Silva, Luiz Henrique Agra; Lettnin, Aline Portantiolo; Votto, Ana Paula de Souza; Vasconcelos, Ulrich; Rodrigues-Mascarenhas, Sandra.
Afiliação
  • Sales-Neto JM; Laboratory of Immunobiotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa, PB, Brazil.
  • da Silva JSF; Laboratory of Immunobiotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa, PB, Brazil.
  • Carvalho DCM; Laboratory of Immunobiotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa, PB, Brazil.
  • Lima ÉA; Laboratory of Immunobiotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa, PB, Brazil.
  • Cavalcante-Silva LHA; Laboratory of Immunobiotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa, PB, Brazil.
  • Lettnin AP; Laboratory of Cell Culture, Biological Sciences Institute, Federal University of Rio Grande, Rio Grande, RS, Brazil.
  • Votto APS; Laboratory of Cell Culture, Biological Sciences Institute, Federal University of Rio Grande, Rio Grande, RS, Brazil.
  • Vasconcelos U; Laboratory of Environmental Microbiology, Biotechnology Center, Federal University of Paraíba, João Pessoa, PB, Brazil.
  • Rodrigues-Mascarenhas S; Laboratory of Immunobiotechnology, Biotechnology Center, Federal University of Paraíba, João Pessoa, PB, Brazil.
Nat Prod Res ; 36(22): 5879-5883, 2022 Nov.
Article em En | MEDLINE | ID: mdl-34963384
Patulin (PAT) is a natural product isolated from several species of fungi. Here, we evaluated the effect of PAT (62.5-4,000 ng/ml) in lipopolysaccharide (LPS)-activated murine peritoneal macrophages. Cell viability assay showed that PAT at concentrations up to 250 ng/ml did not affect macrophage viability. PAT (250 ng/ml) significantly reduced LPS-induced nitric oxide production (by 98.4%), inducible nitric oxide synthase (iNOS) expression (by 83.5%), and iNOS messenger ribonucleic acid expression (by 100.0%). Moreover, PAT significantly reduced LPS-induced interleukin-1ß (by 80.6%), cluster of differentiation (CD) 69 (by 63.1%), and Toll-like receptor (TLR) 4 (by 91.9%) protein expression. Finally, PAT significantly reduced LPS-triggered phosphorylation of all mitogen-activated protein kinases (MAPK) assessed: extracellular signal-regulated kinase (ERK; by 89.5%), c-Jun N-terminal kinase (JNK; by 77.5%), and p38 (by 72.3%). Taken together, these data suggest that PAT downregulates acute inflammatory response, inhibiting nitric oxide production by suppressing CD69-TLR4/ERK-JNK-p38 MAPKs/Nos2/iNOS signaling pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Patulina / Lipopolissacarídeos Limite: Animals Idioma: En Revista: Nat Prod Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Patulina / Lipopolissacarídeos Limite: Animals Idioma: En Revista: Nat Prod Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido