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Performance of microvesicles as biomarkers of clinical outcome in sepsis and trauma: A pilot study.
Puga, Marcelo Lourencini; Menegueti, Mayra Gonçalves; Silvestrini, Marina Malheiros Araújo; de Souza Santos, Lorena Júnia; Ferreira-Nogueira, Raquel; Basile-Filho, Anibal; Teixeira-Carvalho, Andréa; Martins-Filho, Olindo Assis; Auxiliadora-Martins, Maria.
Afiliação
  • Puga ML; Division of Intensive Care, Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
  • Menegueti MG; Ribeirão Preto Nursing School, University of São Paulo, Ribeirão Preto, Brazil.
  • Silvestrini MMA; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ, Minas, Brazil.
  • de Souza Santos LJ; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ, Minas, Brazil.
  • Ferreira-Nogueira R; Beckman Coulter, Brazil.
  • Basile-Filho A; Division of Intensive Care, Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
  • Teixeira-Carvalho A; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ, Minas, Brazil.
  • Martins-Filho OA; Instituto René Rachou, Fundação Oswaldo Cruz, FIOCRUZ, Minas, Brazil. Electronic address: olindo.filho@fiocruz.br.
  • Auxiliadora-Martins M; Division of Intensive Care, Department of Surgery and Anatomy, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil. Electronic address: mamartins@hcrp.usp.br.
Biomed Pharmacother ; 146: 112490, 2022 Feb.
Article em En | MEDLINE | ID: mdl-34891115
Sepsis remains one of the main causes of death in intensive care unit (ICU) worldwide, despite all technological and scientific advances. Microvesicles (MV) have become promising biomarkers for quick and accurate monitoring of several illnesses. The aim of this pilot study was to characterize and evaluate the performance of MV as biomarker of clinical outcome in septic and trauma patients. For this purpose, 39 subjects, both genders, aging from 18 to 85 years were included in three groups referred as Sepsis, Trauma and Healthy Control. Kinetic analysis of MV was carried out at four consecutive time points: admission (baseline)/T1, 24 h/T2, 72 h/T3 and outcome/T4 of discharge or death. At admission, an overall increase in total MV (Annexin V+) was observed in Sepsis.MV CD14+ (monocytes) was a putative biomarker to identify trauma patients, while MV CD3+ (T-cells) and CD41+ (platelets) were qualified to discriminated Trauma from Sepsis. Sepsis (Death) presented an increase in MV Annexin V+, CD45+, CD16+, CD14+, and CD41+ in comparison to Sepsis (Discharge). Moreover, Trauma (Death) presented an increase of MV CD3+ and CD235+ as compared to Trauma (Discharge). Analysing the ROC curve of specific MV evaluated according to performance, an accuracy of 100% was found to segregate the outcome in sepsis, and 95% in trauma. Our findings suggest that MV might be useful as a potential role in discriminating outcome in patients with sepsis/septic shock and trauma with high accuracy. However, further studies with a larger number of participants will be necessary to validate our findings.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ferimentos e Lesões / Biomarcadores / Sepse / Micropartículas Derivadas de Células Tipo de estudo: Observational_studies / Prognostic_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: França
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ferimentos e Lesões / Biomarcadores / Sepse / Micropartículas Derivadas de Células Tipo de estudo: Observational_studies / Prognostic_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: França