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Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars.
Di Pauli, Valentina; Fontana, Paola Daniela; Lewi, Dalia Marcela; Felipe, Arturo; Erazzú, Luis Ernesto.
Afiliação
  • Di Pauli V; National Institute of Agricultural Technology (INTA), Experimental Station Famaillá, Tucumán, Argentina. dipauli.valentina@inta.gob.ar.
  • Fontana PD; National Institute of Agricultural Technology (INTA), Experimental Station Famaillá, Tucumán, Argentina.
  • Lewi DM; National Institute of Agricultural Technology (INTA), Genetic Institute Ewald A. Favret (IGEAF), Buenos Aires, Argentina.
  • Felipe A; National Institute of Agricultural Technology (INTA), Experimental Station Famaillá, Tucumán, Argentina.
  • Erazzú LE; National Institute of Agricultural Technology (INTA), Experimental Station Famaillá, Tucumán, Argentina.
J Genet Eng Biotechnol ; 19(1): 171, 2021 Nov 08.
Article em En | MEDLINE | ID: mdl-34750689
BACKGROUND: Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, which are adapted to Argentinian agro-ecological conditions, are capable of being regenerated via indirect somatic embryogenesis. Leaf rolls of five elite genotypes were cultured following two callus induction protocols using different concentrations of 2,4-D as the growth regulator. Embryogenic calluses were regenerated under light conditions. Regenerated plants were subsequently acclimatized in the greenhouse under two acclimatization procedures before being transplanted to the field. RESULTS: Four of the five genotypes were able to form somatic embryos following the two induction protocols. The variables related to embryogenic callus production were influenced by the interaction between genotype and culture conditions. For plant regeneration, the embryogenic calluses were further cultured on an IBA-supplemented medium, where we observed a high genotype dependence. Calluses from the four cultivars regenerated a good number of plants. With the procedures described here, we obtained more than 90% of well-acclimatized plants both in the greenhouse and in the field. CONCLUSIONS: This protocol provides a simple way to regenerate sugarcane plants through indirect somatic embryogenesis. Also, the results confirm that tissue culture ability is highly genotype-dependent in sugarcane. Our findings suggest that these elite cultivars could be good candidates for biotechnological breeding.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: America do sul / Argentina Idioma: En Revista: J Genet Eng Biotechnol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: America do sul / Argentina Idioma: En Revista: J Genet Eng Biotechnol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Holanda