Occurrence of the Seven Most Common Serotypes of Shiga Toxin-Producing Escherichia coli in Beef Cuts Produced in Meat Processing Plants in the State of São Paulo, Brazil.
J Food Prot
; 85(2): 261-265, 2022 02 01.
Article
em En
| MEDLINE
| ID: mdl-34706050
ABSTRACT: Healthy cattle are considered the main reservoir of Shiga toxin-producing Escherichia coli (STEC) strains, so in some places in the world, products derived from beef are the most common source for disease outbreaks caused by these bacteria. Therefore, to guarantee that the beef produced by our slaughterhouses is safe, there is a need for continuous monitoring of these bacteria. In this study, 215 beef cuts were evaluated, including chilled vacuum-packed striploins (151 samples), rib eyes (30 samples), and knuckles (34 samples), from March to June 2018. These meat samples were collected from the slaughter of unconfined cattle, being arbitrarily collected from eight meat processing companies in São Paulo state, Brazil. Each sample was examined for the presence of STEC toxin type (stx1 and/or stx2 genes) and also the attaching and effacing E. coli (eae) gene, determined by a multiplex PCR assay. We show that the major seven STEC strains (O serogroups O26, O45, O103, O111, O121, O145, and O157) are not detected in any of the analyzed beef cut samples; however, three of them presented the virulence eae gene. Therefore, the absence of STEC strains in the beef samples may be an indication of the low prevalence of this pathogen in the cattle herd on the farm, associated with good hygiene and handling practices adopted by the meat industry.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Escherichia coli
/
Escherichia coli Shiga Toxigênica
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Food Prot
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos