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Docetaxel and Lidocaine Co-Loaded (NLC-in-Hydrogel) Hybrid System Designed for the Treatment of Melanoma.
de Moura, Ludmilla David; Ribeiro, Lígia N M; de Carvalho, Fabíola V; Rodrigues da Silva, Gustavo H; Lima Fernandes, Priscila C; Brunetto, Sérgio Q; Ramos, Celso D; Velloso, Lício A; de Araújo, Daniele R; de Paula, Eneida.
Afiliação
  • de Moura LD; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas-UNICAMP, Campinas 13083-862, SP, Brazil.
  • Ribeiro LNM; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas-UNICAMP, Campinas 13083-862, SP, Brazil.
  • de Carvalho FV; Institute of Biotechnology, Federal University of Uberlândia-UFU, Uberlândia 38405-319, MG, Brazil.
  • Rodrigues da Silva GH; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas-UNICAMP, Campinas 13083-862, SP, Brazil.
  • Lima Fernandes PC; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas-UNICAMP, Campinas 13083-862, SP, Brazil.
  • Brunetto SQ; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas-UNICAMP, Campinas 13083-862, SP, Brazil.
  • Ramos CD; Radiology Department, University of Campinas-UNICAMP, Campinas 13083-887, SP, Brazil.
  • Velloso LA; Radiology Department, University of Campinas-UNICAMP, Campinas 13083-887, SP, Brazil.
  • de Araújo DR; Clinical Medicine Department, School of Medicine Science, University of Campinas-UNICAMP, Campinas 13083-887, SP, Brazil.
  • de Paula E; Human and Natural Science Center, ABC Federal University-UFABC, Santo André 09210-580, SP, Brazil.
Pharmaceutics ; 13(10)2021 Sep 24.
Article em En | MEDLINE | ID: mdl-34683846
Melanoma is the most aggressive skin carcinoma and nanotechnology can bring new options for its pharmacological treatment. Nanostructured lipid carriers (NLC) are ideal drug-delivery carriers for hydrophobic drugs, such as the antineoplastic docetaxel (DTX), and hybrid (NLC-in-hydrogel) systems are suitable for topical application. This work describes a formulation of NLCDTX in xanthan-chitosan hydrogel containing lidocaine (LDC) with anticancer and analgesia effects. The optimized nanoparticles encapsulated 96% DTX and rheological analysis revealed inherent viscoelastic properties of the hydrogel. In vitro assays over murine fibroblasts (NIH/3T3) and melanoma cells (B16-F10), human keratinocytes (HaCaT) and melanoma cells (SK-MEL-103) showed reduction of docetaxel cytotoxicity after encapsulation in NLCDTX and HGel-NLCDTX. Addition of LDC to the hybrid system (HGel-NLCDTX-LDC) increased cell death in tumor and normal cells. In vivo tests on C57BL/6J mice with B16-F10-induced melanoma indicated that LDC, NLCDTX, HGel-NLCDTX-LDC and NLCDTX + HGel-LDC significantly inhibited tumor growth while microPET/SPECT/CT data suggest better prognosis with the hybrid treatment. No adverse effects were observed in cell survival, weight/feed-consumption or serum biochemical markers (ALT, AST, creatinine, urea) of animals treated with NLCDTX or the hybrid system. These results confirm the adjuvant antitumor effect of lidocaine and endorse HGel-NLCDTX-LDC as a promising formulation for the topical treatment of melanoma.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pharmaceutics Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pharmaceutics Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça