Mathematical Description of the Enzymatic Activity of Proteins with Ionizable Groups Exhibiting Deviations from the Henderson-Hasselbalch Equation.

Franco, Luis Fernando Mercier; Pessoa Filho, Pedro de Alcantara

Franco, Luis Fernando Mercier; Pessoa Filho, Pedro de Alcantara.

Afiliação

Franco LFM; Chemical Engineering School, University of Campinas, Av. Albert Einstein, 500, Campinas, SP, 13083-852, Brazil.
Pessoa Filho PA; Department of Chemical Engineering, Engineering School, University of Sao Paulo, Av. Prof. Lineu Prestes, 580, Bl. 20, Sao Paulo, SP, 05508-000, Brazil. pedro.pessoa@poli.usp.br.

Appl Biochem Biotechnol ; 194(3): 1221-1234, 2022 Mar.

Article em En | MEDLINE | ID: mdl-34652586

RESUMO

The ionization equilibrium implied in the calculation of the specific activity is classically described through the Henderson-Hasselbalch equation. An extension for the description of anomalous ionization profiles using the Hill equation is presented in this communication. The proposed framework was applied to the description of the specific enzymatic activity curve as a function of pH of five enzymes presenting different ionization states in their active site. The developed equation improves the description of relative enzymatic curves that deviate from the bell curve predicted by the application of the Henderson-Hasselbalch equation, regardless of the ionization scheme related to the active site.

Assuntos

Eletricidade Estática

Palavras-chave

Activity; Enzyme; Henderson-Hasselbalch; Model; Titration

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Eletricidade Estática Tipo de estudo: Prognostic_studies Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

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