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Analytical Platforms for Mass Spectrometry-Based Metabolomics of Polar and Ionizable Metabolites.
Macedo, Adriana N; Faccio, Andrea T; Fukuji, Tatiana S; Canuto, Gisele A B; Tavares, Marina F M.
Afiliação
  • Macedo AN; Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. adrianamacedo@qui.ufmg.br.
  • Faccio AT; Institute of Chemistry, University of Sao Paulo, Sao Paulo, SP, Brazil.
  • Fukuji TS; Institute of Chemistry, University of Sao Paulo, Sao Paulo, SP, Brazil.
  • Canuto GAB; Department of Analytical Chemistry, Institute of Chemistry, Federal University of Bahia, Salvador, BA, Brazil.
  • Tavares MFM; Institute of Chemistry, University of Sao Paulo, Sao Paulo, SP, Brazil.
Adv Exp Med Biol ; 1336: 215-242, 2021.
Article em En | MEDLINE | ID: mdl-34628634
Metabolomics studies rely on the availability of suitable analytical platforms to determine a vast collection of chemically diverse metabolites in complex biospecimens. Liquid chromatography-mass spectrometry operated under reversed-phase conditions is the most commonly used platform in metabolomics, which offers extensive coverage for nonpolar and moderately polar compounds. However, complementary techniques are required to obtain adequate separation of polar and ionic metabolites, which are involved in several fundamental metabolic pathways. This chapter focuses on the main mass-spectrometry-based analytical platforms used to determine polar and/or ionizable compounds in metabolomics (GC-MS, HILIC-MS, CE-MS, IPC-MS, and IC-MS). Rather than comprehensively describing recent applications related to GC-MS, HILIC-MS, and CE-MS, which have been covered in a regular basis in the literature, a brief discussion focused on basic principles, main strengths, limitations, as well as future trends is presented in this chapter, and only key applications with the purpose of illustrating important analytical aspects of each platform are highlighted. On the other hand, due to the relative novelty of IPC-MS and IC-MS in the metabolomics field, a thorough compilation of applications for these two techniques is presented here.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Redes e Vias Metabólicas / Metabolômica Idioma: En Revista: Adv Exp Med Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Redes e Vias Metabólicas / Metabolômica Idioma: En Revista: Adv Exp Med Biol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos