Your browser doesn't support javascript.
loading
Comparative performance and reusability studies of lipases on syntheses of octyl esters with an economic approach.
de Sousa, Ronaldo Rodrigues; Pinto, Martina Costa Cerqueira; Aguieiras, Erika Cristina Gonçalves; Cipolatti, Eliane Pereira; Manoel, Evelin Andrade; da Silva, Ayla Sant'Ana; Pinto, José Carlos; Freire, Denise Maria Guimarães; Ferreira-Leitão, Viridiana Santana.
Afiliação
  • de Sousa RR; Biocatalysis Laboratory, Ministry of Science, Technology, and Innovations, National Institute of Technology, Rio de Janeiro, RJ, 20081-312, Brazil.
  • Pinto MCC; Department of Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
  • Aguieiras ECG; Federal University of Rio de Janeiro, Chemical Engineering Program, COPPE, Rio de Janeiro, RJ, 21941-972, Brazil.
  • Cipolatti EP; Department of Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
  • Manoel EA; Department of Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
  • da Silva AS; Federal University of Rio de Janeiro Campus, UFRJ - Duque de Caxias, Prof. Geraldo Cidade, Duque de Caxias, RJ, 25240-005, Brazil.
  • Pinto JC; Department of Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
  • Freire DMG; Pharmaceutical Biotechnology Program, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-972, Brazil.
  • Ferreira-Leitão VS; Pharmaceutical Biotechnology Program, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-972, Brazil.
Bioprocess Biosyst Eng ; 45(1): 131-145, 2022 Jan.
Article em En | MEDLINE | ID: mdl-34605995
A suitable immobilized lipase for esters syntheses should be selected considering not only its cost. We evaluated five biocatalysts in syntheses of octyl caprylate, octyl caprate, and octyl laurate, in which conversions higher than 90% were achieved. Novozym®ï»¿ 435 and non-commercial preparations (including a dry fermented solid) were selected for short-term octyl laurate syntheses using different biocatalysts loadings. By increasing the biocatalyst's loading the lipase's reusability also raised, but without strict proportionality, which resulted in a convergence between the lowest biocatalyst loading and the lowest cost per batch. The use of a dry fermented solid was cost-effective, even using loadings as high as 20.0% wt/wt due to its low obtaining cost, although exhibiting low productiveness. The combination of biocatalyst's cost, esterification activity, stability, and reusability represents proper criteria for the choice. This kind of assessment may help to establish quantitative goals to improve or to develop new biocatalysts.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enzimas Imobilizadas / Lipase Tipo de estudo: Health_economic_evaluation Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enzimas Imobilizadas / Lipase Tipo de estudo: Health_economic_evaluation Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha