Your browser doesn't support javascript.
loading
Biophysical properties of electrospun chitosan-grafted poly(lactic acid) nanofibrous scaffolds loaded with chondroitin sulfate and silver nanoparticles.
Júnior, Alexandre F; Ribeiro, Charlene A; Leyva, Maria E; Marques, Paulo S; Soares, Carlos R J; Alencar de Queiroz, Alvaro A.
Afiliação
  • Júnior AF; Doctorate Post-graduate scholarship in Materials for Engineering/Biomaterials (CAPES), 28094Federal University of Itajubá (UNIFEI), Itajubá, Brazil.
  • Ribeiro CA; Doctorate Post-graduate scholarship in Materials for Engineering/Biomaterials (CAPES), 28094Federal University of Itajubá (UNIFEI), Itajubá, Brazil.
  • Leyva ME; 28094Institute of Physics and Chemistry/Federal University of Itajubá (UNIFEI), Itajubá, Brazil.
  • Marques PS; 28094Institute of Natural Resources (IRN)/Federal University of Itajubá (UNIFEI), Itajubá, Brazil.
  • Soares CRJ; Biotechnology Center (CEBIO), 119500Nuclear and Energy Research Institute, Sao Paulo, Brazil.
  • Alencar de Queiroz AA; Biotechnology Center (CEBIO), 119500Nuclear and Energy Research Institute, Sao Paulo, Brazil.
J Biomater Appl ; 36(6): 1098-1110, 2022 01.
Article em En | MEDLINE | ID: mdl-34601887
The aim of this work was to study the biophysical properties of the chitosan-grafted poly(lactic acid) (CH-g-PLA) nanofibers loaded with silver nanoparticles (AgNPs) and chondroitin-4-sulfate (C4S). The electrospun CH-g-PLA:AgNP:C4S nanofibers were manufactured using the electrospinning technique. The microstructure of the CH-g-PLA:AgNP:C4S nanofibers was investigated by proton nuclear magnetic resonance (1H-NMR), scanning electron microscopy (SEM), UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD), and Fourier transform infrared (ATR-FTIR) spectroscopy. ATR-FTIR and 1H-NMR confirm the CH grafting successfully by PLA with a substitution degree of 33.4%. The SEM measurement results indicated apparently smooth nanofibers having a diameter range of 340 ± 18 nm with porosity of 89 ± 3.08% and an average pore area of 0.27 µm2. UV-Vis and XRD suggest that silver nanoparticles with the size distribution of 30 nm were successfully incorporated into the electrospun nanofibers. The water contact angle of 12.8 ± 2.7° reveals the hydrophilic nature of the CH-g-PLA:AgNP:C4S nanofibers has been improved by C4S. The electrospun CH-g-PLA:AgNP:C4S nanofibers are found to release ions Ag+ at a concentration level capable of rendering an antimicrobial efficacy. Gram-positive bacteria (S.aureus) were more sensitive to CH-g-PLA:AgNP:C4S than Gram-negative bacteria (E. coli). The electrospun CH-g-PLA:AgNP:C4S nanofibers exhibited no cytotoxicity to the L-929 fibroblast cells, suggesting cytocompatibility. Fluorescence microscopy demonstrated that C4S promotes the adhesion and proliferation of fibroblast cells onto electrospun CH-g-PLA:AgNP:C4S nanofibers.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quitosana / Nanopartículas Metálicas / Nanofibras Idioma: En Revista: J Biomater Appl Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quitosana / Nanopartículas Metálicas / Nanofibras Idioma: En Revista: J Biomater Appl Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido