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Metagenomic Analysis of Biochemical Passive Reactors During Acid Mine Drainage Bioremediation Reveals Key Co-selected Metabolic Functions.
Villegas-Plazas, Marcela; Sanabria, Janeth; Arbeli, Ziv; Vasquez, Yaneth; Roldan, Fabio; Junca, Howard.
Afiliação
  • Villegas-Plazas M; Environmental Microbiology and Biotechnology Laboratory, Engineering School of Environmental & Natural Resources, Engineering Faculty, Universidad del Valle, Cali, Colombia.
  • Sanabria J; RG Microbial Ecology: Metabolism, Genomics & Evolution, Div. Ecogenomics & Holobionts, Microbiomas Foundation, LT11A, 250008, Chia, Colombia.
  • Arbeli Z; Environmental Microbiology and Biotechnology Laboratory, Engineering School of Environmental & Natural Resources, Engineering Faculty, Universidad del Valle, Cali, Colombia.
  • Vasquez Y; Departamento de Biología, Unidad de Saneamiento y Biotecnología Ambiental USBA, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Roldan F; Departamento de Ciencias Naturales, Facultad de Ingeniería y Ciencias Básicas, Universidad Central, Carrera 5 No. 21-38, Bogotá, Colombia.
  • Junca H; Departamento de Biología, Unidad de Saneamiento y Biotecnología Ambiental USBA, Pontificia Universidad Javeriana, Bogotá, Colombia.
Microb Ecol ; 84(2): 465-472, 2022 Aug.
Article em En | MEDLINE | ID: mdl-34591135
Acid mine drainage (AMD) is the major pollutant generated by the mining industry, and it is characterized by low pH and high concentration of metals and sulfate. The use of biochemical passive reactors (BPRs) is a promising strategy for its bioremediation. To date, there are various studies describing the taxonomical composition of BPR microbial communities, generally consisting of an assemblage of sulfate-reducing organisms inside Deltaproteobacteria, and a diverse set of anaerobic (ligno)cellulolytic bacteria; however, insights about its functional metagenomic content are still scarce. In previous studies, a laboratory-scale AMD bioremediation using biochemical passive reactors was designed and performed, tracking operation parameters, chemical composition, and changes, together with taxonomic composition of the microbiomes harbored in these systems. In order to reveal the main functional content of these communities, we used shotgun metagenomics analyses to explore genes of higher relative frequencies and their inferred functions during the AMD bioremediation from three BPRs representing the main microbiome compositions detected in the system. Remarkably, genes encoding for two-component regulatory systems and ABC transporters related to metal and inorganic ions, cellulose degradation enzymes, dicarboxylic acid production, and sulfite reduction complex were all detected at increased frequency. Our results evidenced that higher taxonomic diversity of the microbiome was arising together with a functional redundancy of the specific metabolic roles, indicating its co-selection and suggesting that its enrichment on BPRs may be implicated in the cumulative efficiency of these systems.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metagenômica / Mineração Idioma: En Revista: Microb Ecol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Metagenômica / Mineração Idioma: En Revista: Microb Ecol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos