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Characterization of the genetic structure of mcr-1 gene among Escherichia coli isolates recovered from surface waters and sediments from Ecuador.
Calero-Cáceres, William; Tadesse, Daniel; Jaramillo, Katherine; Villavicencio, Xavier; Mero, Efraín; Lalaleo, Liliana; Welsh, Caitlin; Villacís, José E; Quentin, Emmanuelle; Parra, Henry; Ramirez, Maria Soledad; Harries, Anthony D; Balcázar, José L.
Afiliação
  • Calero-Cáceres W; UTA-RAM-One Health, Department of Food and Biotechnology Science and Engineering, Universidad Técnica de Ambato, Ambato, Ecuador; Centro de Investigaciones Agropecuarias, Facultad de Ciencias Agropecuarias, Universidad Técnica de Ambato, Cevallos, Ecuador. Electronic address: wr.calero@uta.edu.ec.
  • Tadesse D; U.S. Food & Drug Administration, Center for Veterinary Medicine, Office of Research Laurel, MD 20708, USA.
  • Jaramillo K; Centro de Referencia Nacional de Resistencia a los Antimicrobianos RAM, Instituto Nacional de Investigación en Salud Pública "Dr. Leopoldo Izquieta Pérez" INSPI, Quito, Ecuador.
  • Villavicencio X; Centro de Referencia Nacional de Resistencia a los Antimicrobianos RAM, Instituto Nacional de Investigación en Salud Pública "Dr. Leopoldo Izquieta Pérez" INSPI, Quito, Ecuador.
  • Mero E; Centro de Investigaciones Agropecuarias, Facultad de Ciencias Agropecuarias, Universidad Técnica de Ambato, Cevallos, Ecuador.
  • Lalaleo L; UTA-RAM-One Health, Department of Food and Biotechnology Science and Engineering, Universidad Técnica de Ambato, Ambato, Ecuador.
  • Welsh C; U.S. Food & Drug Administration, Center for Veterinary Medicine, Office of Research Laurel, MD 20708, USA.
  • Villacís JE; Centro de Referencia Nacional de Resistencia a los Antimicrobianos RAM, Instituto Nacional de Investigación en Salud Pública "Dr. Leopoldo Izquieta Pérez" INSPI, Quito, Ecuador.
  • Quentin E; Centro de Referencia Nacional de Resistencia a los Antimicrobianos RAM, Instituto Nacional de Investigación en Salud Pública "Dr. Leopoldo Izquieta Pérez" INSPI, Quito, Ecuador.
  • Parra H; Centro de Investigación Microbiológica Dr. Henry Parra, Guayaquil, Ecuador.
  • Ramirez MS; Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA, USA.
  • Harries AD; International Union Against Tuberculosis and Lung Disease, Paris, France; London School of Hygiene and Tropical Medicine, London, UK.
  • Balcázar JL; Catalan Institute for Water Research (ICRA), 17003 Girona, Spain; University of Girona, 17004 Girona, Spain.
Sci Total Environ ; 806(Pt 2): 150566, 2022 Feb 01.
Article em En | MEDLINE | ID: mdl-34582864
Although anthropogenic activities contribute to the selection and spread of antibiotic resistance in aquatic environments, limited information is available from countries with absent or incomplete sewage treatment systems and the impact of their discharges onto water bodies. This study therefore aimed to characterize the genetic structure of colistin resistance (mcr) genes among Escherichia coli isolates recovered from surface waters and sediments in Ecuador. Out of 459 isolates, four Escherichia coli showed multidrug-resistant phenotypes, which harbored the mcr-1 gene and ß-lactamases, such as blaTEM, blaCTX-M-15, blaCTX-M-55, or blaCTX-M-65 genes. Three E. coli isolates (U20, U30 and U144) shared a similar genetic environment surrounding the mcr-1 gene, which was located on plasmids. Only one E. coli isolate (U175) showed that the mcr-1 gene was chromosomally located. Moreover, the core genome multilocus sequence typing (cgMLST) analysis revealed that these isolates belong to different lineages. This study represents the first detection of the mcr-1 gene in multidrug-resistant E. coli isolates from environmental samples in Ecuador.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Escherichia coli / Escherichia coli País/Região como assunto: America do sul / Ecuador Idioma: En Revista: Sci Total Environ Ano de publicação: 2022 Tipo de documento: Article País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Escherichia coli / Escherichia coli País/Região como assunto: America do sul / Ecuador Idioma: En Revista: Sci Total Environ Ano de publicação: 2022 Tipo de documento: Article País de publicação: Holanda