Purification and characterization of aminopeptidase activity from rat urine.

Alves, K B; Noguti, M A; Silva, M M; Brandi, C M

Alves, K B; Noguti, M A; Silva, M M; Brandi, C M.

Afiliação

Alves KB; Departamento de Bioquímica, Escola Paulista de Medicina, São Paulo, Brasil.

Braz J Med Biol Res ; 20(6): 759-61, 1987.

Article em En | MEDLINE | ID: mdl-3455254

RESUMO

The aminopeptidase activity of rat urine was tested using L-aminoacyl-2-naphthylamides and L-Leu-p-nitroanilide. The enzyme preparation was purified by ion-exchange and gel filtration chromatography and showed only a single active protein band on 7.5% polyacrylamide gel electrophoresis. The enzyme was characterized by studying the effects of ions (divalent cations and chloride), chelating agents and -SH and -S-S- group reagents and by determining kinetic parameters (Km and Vmax for different substrates and Ki for amino acids, antibiotics and anti-inflammatory drugs).

Assuntos

Aminopeptidases/metabolismo; Aminopeptidases/urina; Aminopeptidases/antagonistas & inibidores; Aminopeptidases/isolamento & purificação; Animais; Cátions Bivalentes/farmacologia; Cinética; Ratos

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aminopeptidases Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 1987 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

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