Purification and characterization of aminopeptidase activity from rat urine.
Braz J Med Biol Res
; 20(6): 759-61, 1987.
Article
em En
| MEDLINE
| ID: mdl-3455254
The aminopeptidase activity of rat urine was tested using L-aminoacyl-2-naphthylamides and L-Leu-p-nitroanilide. The enzyme preparation was purified by ion-exchange and gel filtration chromatography and showed only a single active protein band on 7.5% polyacrylamide gel electrophoresis. The enzyme was characterized by studying the effects of ions (divalent cations and chloride), chelating agents and -SH and -S-S- group reagents and by determining kinetic parameters (Km and Vmax for different substrates and Ki for amino acids, antibiotics and anti-inflammatory drugs).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aminopeptidases
Limite:
Animals
Idioma:
En
Revista:
Braz J Med Biol Res
Ano de publicação:
1987
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil