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Compensatory Hippocampal Neurogenesis in the Absence of Cognitive Impairment Following Experimental Hippocampectomy in Adult Rats.
Cardoso, Giuliana T M; Gomes-Leal, Walace; Franco, Edna C S; Pereira, Antonio; Gomes, Francinaldo L; Brino, Ana Leda F; Lima, Silene M A.
Afiliação
  • Cardoso GTM; Laboratory of Neurobiology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.
  • Gomes-Leal W; Laboratory of Experimental Neuroprotection and Neuroregeneration, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.
  • Franco ECS; Pathology Unit, Evandro Chagas Institute, Ananindeua, Brazil.
  • Pereira A; Laboratory of Signal Processing, Institute of Technology, Federal University of Pará, Belém, Brazil.
  • Gomes FL; Neurology Unit, João de Barros Barreto University Hospital, Federal University of Pará, Belém, Brazil.
  • Brino ALF; Experimental School of Primates, Center for Theory and Behavioral Research, Federal University of Pará, Belém, Brazil.
  • Lima SMA; Laboratory of Neurobiology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.
Front Cell Neurosci ; 15: 709291, 2021.
Article em En | MEDLINE | ID: mdl-34531725
Temporal lobe epilepsy (TLE) is the commonest type of focal epilepsy in adult humans, and hippocampal sclerosis (HS) is the main pathological finding in this type of epilepsy. In refractory TLE, patients are indicated for unilateral resection of the affected hippocampus by a surgical procedure called hippocampectomy which generally does not cause any cognitive impairment. Once adult hippocampus is a region of endogenous neurogenesis, even in elderly people, we have hypothesized that a compensatory increase in hippocampal neurogenesis might occur in the remaining hippocampus after unilateral hippocampectomy. To test this hypothesis, we performed unilateral hippocampectomy in adult Wistar rats, which were perfused at 15 (G15) and 30 (G30) days post-surgery. Eighteen Wistar rats were randomly distributed in the following experimental groups: control (no surgery, N = 6), G15 (N = 6), and G30 (N = 6). Adjacent cortex and hippocampus of the left hemisphere were completely removed. Behavioral procedures were performed to address possible cognitive impairments. Brains were collected and fixed from animals belonging to all experimental groups. Gross histopathology was performed using thionine staining. Neuroblasts and mature neurons were immunolabeled using anti-doublecortin (DCX) and anti-NeuN antibodies, respectively. Numbers of DCX and NeuN positive cells were quantified for all experimental groups. Animals submitted to hippocampectomy did not present any cognitive impairment as evaluated by eight-arm radial maze behavioral test. The remaining hippocampus presented a higher number of DCX positive cells compared to control (p < 0.001, ANOVA-Tukey) at both G15 and G30. A higher number of NeuN positive cells were present in the granular layer of dentate gyrus at G30 compared to control and G15 (p < 0.001, ANOVA-Tukey). The data suggest that unilateral hippocampectomy induces compensatory neurogenic effect in the contralateral hippocampus. This may underlie the reported absence of significant cognitive impairment and parallels the findings in human patients submitted to unilateral hippocampectomy to treat refractory TLE.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Cell Neurosci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Cell Neurosci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça