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Virucidal and antiviral activities of pomegranate (Punica granatum) extract against the mosquito-borne Mayaro virus.
Salles, Tiago Souza; Meneses, Marcelo Damião Ferreira; Caldas, Lucio Ayres; Sá-Guimarães, Thayane Encarnação; de Oliveira, Danielle M; Ventura, José A; Azevedo, Renata Campos; Kuster, Ricardo M; Soares, Márcia Regina; Ferreira, Davis Fernandes.
Afiliação
  • Salles TS; Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. tiagosouzasalles@ufrj.br.
  • Meneses MDF; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. tiagosouzasalles@ufrj.br.
  • Caldas LA; Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Sá-Guimarães TE; National Institute of Science and Technology for Structural Biology and Bioimaging, INBEB, Rio de Janeiro, RJ, Brazil.
  • de Oliveira DM; Laboratory of Cellular Ultrastructure Hertha Meyer, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
  • Ventura JA; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Azevedo RC; Natural Products Research Institute, IPPN, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Kuster RM; Capixaba Institute of Research, Technical Assistance and Rural Extension, Espirito Santo, Vitoria, Brazil.
  • Soares MR; Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Ferreira DF; Natural Products Research Institute, IPPN, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Parasit Vectors ; 14(1): 443, 2021 Sep 03.
Article em En | MEDLINE | ID: mdl-34479605
BACKGROUND: The arthropod-borne Mayaro virus (MAYV) causes "Mayaro fever," a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. There are currently no licensed drugs against most mosquito-transmitted viruses. Punica granatum (pomegranate) fruits cultivated in Brazil have been subjected to phytochemical investigation for the identification and isolation of antiviral compounds. In the present study, we explored the antiviral activity of pomegranate extracts in Vero cells infected with Mayaro virus. METHODS: The ethanol extract and punicalagin of pomegranate were extracted solely from the shell and purified by chromatographic fractionation, and were chemically identified using spectroscopic techniques. The cytotoxicity of the purified compounds was measured by the dye uptake assay, while their antiviral activity was evaluated by a virus yield inhibition assay. RESULTS: Pomegranate ethanol extract (CC50 = 588.9, IC50 = 12.3) and a fraction containing punicalagin as major compound (CC50 = 441.5, IC50 = 28.2) were shown to have antiviral activity (SI 49 and 16, respectively) against Mayaro virus, an alphavirus. Immunofluorescence analysis showed the virucidal effect of pomegranate extract, and transmission electron microscopy (TEM) revealed damage in viral particles treated with this extract. CONCLUSIONS: The P. granatum extract is a promising source of antiviral compounds against the alphavirus MAYV and represents an excellent candidate for future studies with other enveloped RNA viruses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Arbovírus / Replicação Viral / Alphavirus / Compostos Fitoquímicos / Punica granatum / Culicidae Limite: Animals Idioma: En Revista: Parasit Vectors Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antivirais / Arbovírus / Replicação Viral / Alphavirus / Compostos Fitoquímicos / Punica granatum / Culicidae Limite: Animals Idioma: En Revista: Parasit Vectors Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido