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Occurrence of genes associated with virulence in Escherichia coli isolates from chicken carcasses at different stages of processing at a slaughterhouse.
Pinheiro, João Juliano; Gazal, Luiz Eduardo de Souza; de Araujo, Gabriella Oliveira; de Brito, Kelly Cristina Tagliari; Tazzo, Ivonete Fatima; Mesquita, Rafael Tonini; Kobayashi, Renata Katsuko Takayama; Otutumi, Luciana Kazue; Beltrami, José Matheus; Cavalli, Lissandra Souto; de Brito, Benito Guimarães.
Afiliação
  • Pinheiro JJ; Laboratory of Avian Health and Technological Innovation, Institute for Veterinary Research Desidério Finamor (IPVDF), Postgraduate Program in Animal Health, Department of Agricultural Diagnosis and Research, Secretariat of Agriculture, Livestock and Rural Development of Rio Grande Do Sul, Estrada do
  • Gazal LES; Bacteriology Laboratory, Department of Microbiology, Center for Biological Sciences, State University of Londrina, Rodovia Celso Garcia Cid, Km 380, S/N Campus Universitário, Londrina, PR, 86057-970, Brazil.
  • de Araujo GO; Laboratory of Avian Health and Technological Innovation, Institute for Veterinary Research Desidério Finamor (IPVDF), Postgraduate Program in Animal Health, Department of Agricultural Diagnosis and Research, Secretariat of Agriculture, Livestock and Rural Development of Rio Grande Do Sul, Estrada do
  • de Brito KCT; Laboratory of Avian Health and Technological Innovation, Institute for Veterinary Research Desidério Finamor (IPVDF), Postgraduate Program in Animal Health, Department of Agricultural Diagnosis and Research, Secretariat of Agriculture, Livestock and Rural Development of Rio Grande Do Sul, Estrada do
  • Tazzo IF; Department of Agricultural Diagnosis and Research, Secretariat of Agriculture, Livestock and Rural Development of Rio Grande do Sul, Rua Gonçalves Dias, 570, Menino Deus, Porto Alegre, RS, 90130-060, Brazil.
  • Mesquita RT; Laboratory of Avian Health and Technological Innovation, Institute for Veterinary Research Desidério Finamor (IPVDF), Postgraduate Program in Animal Health, Department of Agricultural Diagnosis and Research, Secretariat of Agriculture, Livestock and Rural Development of Rio Grande Do Sul, Estrada do
  • Kobayashi RKT; Bacteriology Laboratory, Department of Microbiology, Center for Biological Sciences, State University of Londrina, Rodovia Celso Garcia Cid, Km 380, S/N Campus Universitário, Londrina, PR, 86057-970, Brazil.
  • Otutumi LK; Postgraduate Program in Animal Science With Emphasis On Bioactive Products, Universidade Paranaense, Umuarama, PR, Brazil.
  • Beltrami JM; Master in Animal Science, Universidade Paranaense, UNIPAR, Umuarama, PR, Brazil.
  • Cavalli LS; Department of Agricultural Diagnosis and Research, Secretariat of Agriculture, Livestock and Rural Development of Rio Grande do Sul, Rua Gonçalves Dias, 570, Menino Deus, Porto Alegre, RS, 90130-060, Brazil.
  • de Brito BG; Laboratory of Avian Health and Technological Innovation, Institute for Veterinary Research Desidério Finamor (IPVDF), Postgraduate Program in Animal Health, Department of Agricultural Diagnosis and Research, Secretariat of Agriculture, Livestock and Rural Development of Rio Grande Do Sul, Estrada do
Braz J Microbiol ; 52(4): 2413-2420, 2021 Dec.
Article em En | MEDLINE | ID: mdl-34467469
Escherichia coli is a bacterium frequently found in chicken carcasses, causing carcass condemnation with losses to the industry and when present in food, it carries a risk to public health as there is evidence that some strains pathogenic to birds (APEC - Avian Pathogenic E. coli) have zoonotic potential. Carcass contamination can occur at the slaughterhouse, but the influence of the different stages of processing in the selection of potential extraintestinal pathogenic E. coli strains is unknown. This study aimed to analyze the influence of the processing steps in the slaughterhouse on the detection of E. coli isolates carrying APEC predictor's virulence-associated genes (VAGs), and to relate their presence with post-mortem condemnation. A sample consisted of four pooled carcasses collected at seven different stages of slaughter (before scalding, after scalding, after plucking, before evisceration/after shower wash, after evisceration, after pre-coolers, and after packing) from 15 batches of broilers. The total samples obtained was 105 pools with four carcasses each, totaling 420 carcasses analyzed. Enterobacteriaceae were counted from each pool and E. coli were subsequently selected, which were submitted to pentaplex PCR to identify the five VAG APEC predictor's: iroN, ompT, hlyF, iss, and iutA. The Enterobacteriaceae count demonstrated a reduction of 4.25 log CFU per gram of carcass from the first to the last stage analyzed, with scalding and pre-cooling by immersion being the procedures that contributed most to this reduction. The presence of VAGs and potential APEC (presence of two or more of these gene predictors) was observed at all points evaluated in the slaughterhouse, which suggested that bacteria carrying these genes could reach the consumer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Virulência / Galinhas / Escherichia coli / Infecções por Escherichia coli Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Braz J Microbiol Ano de publicação: 2021 Tipo de documento: Article País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Virulência / Galinhas / Escherichia coli / Infecções por Escherichia coli Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Braz J Microbiol Ano de publicação: 2021 Tipo de documento: Article País de publicação: Brasil