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Validation of Reference Genes for Quantitative PCR in Johnsongrass (Sorghum halepense L.) under Glyphosate Stress.
Ulrich, María Noelia; Muñiz-Padilla, Esteban; Corach, Alejandra; Hopp, Esteban; Tosto, Daniela.
Afiliação
  • Ulrich MN; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de investigaciones Científicas y Tecnológicas (CONICET), Nicolás Repetto y de los Reseros S/N, Hurlingham B1686IGC, Argentina.
  • Muñiz-Padilla E; Facultad de Ciencias Agropecuarias, Universidad Nacional de Entre Ríos/Facultad de Ciencia y Tecnología, Universidad Autónoma de Entre Ríos, Ruta 11 km 10.5, Oro Verde ER E3100XAD, Argentina.
  • Corach A; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de investigaciones Científicas y Tecnológicas (CONICET), Nicolás Repetto y de los Reseros S/N, Hurlingham B1686IGC, Argentina.
  • Hopp E; Departmento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires C1428EGA, Argentina.
  • Tosto D; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de investigaciones Científicas y Tecnológicas (CONICET), Nicolás Repetto y de los Reseros S/N, Hurlingham B1686IGC, Argentina.
Plants (Basel) ; 10(8)2021 Jul 28.
Article em En | MEDLINE | ID: mdl-34451600
Weeds are one of the main causes of the decrease in crop yields, with Johnsongrass (Sorghum halepense L.) being one of the most significant. Weeds can be controlled by herbicides, but some have developed resistance. Quantitative PCR is the technique of choice for studying gene expression related to herbicide resistance because of its high sensitivity and specificity, although its quantitative accuracy is highly dependent on the stability of the reference genes. Thus, in this study we evaluated the stability of different reference genes of glyphosate-resistant S. halepense. Nine genes frequently used as reference genes were selected: MDH, ADP, PP2A, EIF4α, ACT, ARI8, DnaJ, Hsp70, and ALS1, and their expression analyzed in susceptible and resistant biotypes at 0, 24 and 72 h post-application of glyphosate. The stability was analyzed with the geNorm, NormFinder, and BestKeeper software programs and using the ΔCt method. RefFinder was used to generate a comprehensive stability ranking. The results showed that PP2A and ARI8 were the most stable genes under the test conditions. EPSPS expression was also verified against the best two and the worst two reference genes. This study provides useful information for gene expression analysis under glyphosate stress and will facilitate resistance mechanism studies in this weed species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Plants (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Suíça
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Plants (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Argentina País de publicação: Suíça