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Functionally Active Microbiome in Supragingival Biofilms in Health and Caries.
Corralo, Daniela Jorge; Ev, Laís Daniela; Damé-Teixeira, Nailê; Maltz, Marisa; Arthur, Rodrigo Alex; Do, Thuy; Fatturi Parolo, Clarissa Cavalcanti.
Afiliação
  • Corralo DJ; PhD by Faculty of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Passo Fundo University (UPF), Passo Fundo, Brazil.
  • Ev LD; Department of Social and Preventive Dentistry, Faculty of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
  • Damé-Teixeira N; Department of Dentistry, School of Health Sciences, University of Brasília, Federal District, Brasília, Brazil.
  • Maltz M; Division of Oral Biology, School of Dentistry, University of Leeds, Leeds, United Kingdom.
  • Arthur RA; Department of Social and Preventive Dentistry, Faculty of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
  • Do T; Department of Social and Preventive Dentistry, Faculty of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
  • Fatturi Parolo CC; Division of Oral Biology, School of Dentistry, University of Leeds, Leeds, United Kingdom.
Caries Res ; 55(6): 603-616, 2021.
Article em En | MEDLINE | ID: mdl-34380135
The oral microbiome is unique at inter and intra-individual levels at various sites due to physical and biological factors. This study aimed to compare the bacterial composition of supragingival biofilms collected from enamel sites with different caries activity, from active and inactive-caries subjects, and from caries-free (CF) subjects. Twenty-two individuals (aged between 13 and 76 years old; med = 23.5 years old) were allocated into 3 groups: caries-active (CA) (n = 10), caries-inactive (CI) (n = 6), and CF (n = 6). From the CA group, 3 sites were sampled: CA (active non-cavitated lesion), CI (inactive non-cavitated lesion), and sound enamel surface (S). From the subjects of the CI group, biofilm from a CI lesion was collected (INCL), while for the CF subjects, a pool of biofilm from sound enamel surfaces was sampled. The total RNA was extracted, and cDNA libraries were prepared and paired-end sequenced (Illumina HiSeq 3,000). Final dental biofilm samples analysed from CA was 16 (ANCL-CA = 6, INCL-CA = 4, S-CA = 6); from CI, 3 (INCL-CI = 3); and from CF, 6 (S-CF = 6) (some samples were lost by insufficient genetic material). Read sequences were processed and analysed using the Metagenomics RAST server. High-quality sequences (3,542,190) were clustered into operational taxonomic units (97% identity; SILVA SSU), representing 915 genera belonging to 29 phyla (higher abundant: Actinobacteria, Firmicutes, Bacteroidetes, and Fusobacteria). The presence of a core microbiome was observed (123 shared genera). The alpha diversity analysis showed less bacterial diversity in disease (S-CA) compared to health (S-CF). The dominant genera included Actinomyces, Corynebacterium, Capnocytophaga, Leptotrichia, Veillonella, Prevotella, Streptococcus, Eubacterium, and Neisseria. Veillonella and Leptotrichia were related with disease and Prevotella with health. Corynebacterium, Capnocytophaga, and Actinomyces clustered together presenting high abundance in health and disease. The Metric Multidimensional Scaling Ordination analysis shows that sites from active subjects (ANCL-CA, INCL-CA, and S-CA) are closer to each other than either INCL-CI subjects or S-CF subjects. In conclusion, supragingival bacterial communities presented intra-individual similarities, but inter-individual diversity and difference in bacterial composition reveal that the subject's caries activity status matters more than sites.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cárie Dentária / Microbiota Limite: Adolescent / Adult / Aged / Humans / Middle aged Idioma: En Revista: Caries Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cárie Dentária / Microbiota Limite: Adolescent / Adult / Aged / Humans / Middle aged Idioma: En Revista: Caries Res Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça