Your browser doesn't support javascript.
loading
PPARGC1A promoter DNA-methylation level and glucose metabolism in Ecuadorian women with Turner syndrome.
Álvarez-Nava, Francisco; Salinas, Marco; Bastidas, Daniela; Vicuña, Yosselin; Racines-Orbe, Marcia.
Afiliação
  • Álvarez-Nava F; Biological Sciences School, Faculty of Biological Sciences, Central University of Ecuador, QuitoEcuador.
  • Salinas M; Institute of Biomedicine Research, Central University of Ecuador, Quito, Ecuador.
  • Bastidas D; Biological Sciences School, Faculty of Biological Sciences, Central University of Ecuador, QuitoEcuador.
  • Vicuña Y; Institute of Biomedicine Research, Central University of Ecuador, Quito, Ecuador.
  • Racines-Orbe M; Institute of Biomedicine Research, Central University of Ecuador, Quito, Ecuador.
Horm Mol Biol Clin Investig ; 42(2): 159-165, 2020 Dec 22.
Article em En | MEDLINE | ID: mdl-34332520
OBJECTIVES: Reduced gene expression of PPARGC1A in subjects with insulin resistance (IR) has been reported. Insulin resistance occurs early on the course of Turner syndrome (TS). The main objective of this study was to evaluate the relationship between PPARGC1A promoter DNA methylation status in lymphocytes and insulin sensitivity and secretion in Ecuadorian females with TS. METHODS: We examined a cohort of 34 Ecuadorian patients with TS along with a sex-, age- and BMI-matched reference group. All subjects received a standard 75 g oral glucose tolerance test. Insulin resistance and secretion indices were calculated. The PPARGC1A methylated DNA/unmethylated DNA ratio and mitochondrial content (mtDNA/nDNA ratio) were further determined. RESULTS: Notably, the PPARGC1A DNA methylation level was significantly higher in TS subjects than the reference group and correlated with IR indices. Conversely, mitochondrial content was significantly lower in the study group than healthy controls and negatively correlated with the PPARGC1A methylated DNA/unmethylated DNA ratio in TS individuals. PPARGC1A promoter DNA methylation status contributed to 20% of the total variability in Homeostasis Model Assessment for Insulin Resistance (HOMA-IR) independently of BMI or age in TS subjects. CONCLUSIONS: Our collective findings suggest that expression of PPARGC1A and lower mitochondrial number affect the metabolic phenotype in TS subjects.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Síndrome de Turner / Regulação da Expressão Gênica / Regiões Promotoras Genéticas / Metilação de DNA / Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo / Glucose Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans País/Região como assunto: America do sul / Ecuador Idioma: En Revista: Horm Mol Biol Clin Investig Ano de publicação: 2020 Tipo de documento: Article País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Síndrome de Turner / Regulação da Expressão Gênica / Regiões Promotoras Genéticas / Metilação de DNA / Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo / Glucose Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans País/Região como assunto: America do sul / Ecuador Idioma: En Revista: Horm Mol Biol Clin Investig Ano de publicação: 2020 Tipo de documento: Article País de publicação: Alemanha