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Biogenic and characterizations of new silver nanoparticles stabilized with indole acetic acid derived from Azospirillum brasilense MMGH-SADAT1, their bioactivity, and histopathological assessment in rats.
Salah Abdel-Hamid, Marwa; El Morsy El Wakeel, Mohamed; Hamza, Hanafy Ahmed; Tahoun, Enas A; M Alshehrei, Fatimah; Rizwan, Muhammad; Badawy, Ghada A.
Afiliação
  • Salah Abdel-Hamid M; Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt. Electronic address: marwa.salah@gebri.usc.edu.eg.
  • El Morsy El Wakeel M; Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.
  • Hamza HA; Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.
  • Tahoun EA; Department of Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Menofia, Egypt.
  • M Alshehrei F; Department of Biology, Jumum College University, Umm Al-Qura University, P.O Box 7388, Makkah, 21955, Saudi Arabia.
  • Rizwan M; Department of Environmental Sciences & Engineering, Government College University Faisalabad, Pakistan. Electronic address: mrazi1532@yahoo.com.
  • Badawy GA; Department of Botany, Faculty of Science, El-Fayoum University, Egypt; Department of Biology, University College of Umluj, Umluj Branch University of Tabouk, Saudi Arabia.
Ecotoxicol Environ Saf ; 222: 112521, 2021 Oct 01.
Article em En | MEDLINE | ID: mdl-34274835
An Egyptian rhizobacterium Azospirillum sp. isolated from Sadat city was able to produce indole acetic acid (IAA) up to (30.59 µg/ml). The isolate was identified biochemically and by 16S rRNA sequencing which showed 99.9% similarity to Azospirillum brasilense. The new isolate has been registered in Genbank with accession number MH179119.1. Extracted IAA was used as reducing or stabilizing agent of sliver nanoparticles (AgNPs). Successful fabrication of biogenic IAA-AgNPs was confirmed by Fourier Transform Infrared Spectrophotometer (FTIR) analysis of IAA which showed absorbance peak at 3434.78 cm-1 due to the N-H stretch of primary amines. Highly resolution Transmission Electron Microscopy (HR-TEM) showed AgNPs coating or capping with IAA in spherical shaped with size ranged from 6.01 to 44.02 nm. Energy dispersive X-ray (EDX) analysis revealed that Ag+ ions were attached to the surface of IAA-AgNPs particles. HR-TEM examination showed cell wall damage of Citrobacter freundii cells after exposure to IAA-AgNPs leading to cell death. In vivo results showed that C. freundii infection of rats induced significant increase in liver and kidney functions and deleterious histopathological alteration in rat's tissues. However, treatment by extracted IAA and IAA-AgNPs could normalize the biochemical and histopathological alterations occurred in infected rats. This is the first study to prove that IAA extracted from Azospirillum brasilense is a hopeful capping agent for NPs which has potential to protect against pathogenic infections, nontoxic and/or safe on rat's metabolisms.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Azospirillum brasilense / Nanopartículas Metálicas Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2021 Tipo de documento: Article País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Azospirillum brasilense / Nanopartículas Metálicas Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Ecotoxicol Environ Saf Ano de publicação: 2021 Tipo de documento: Article País de publicação: Holanda