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|Isolation and characterization of novel bacteriophages as a potential therapeutic option for Escherichia coli urinary tract infections.
González-Villalobos, Edgar; Ribas-Aparicio, Rosa María; Montealegre, Gerardo Erbey Rodea; Belmont-Monroy, Laura; Ortega-García, Yerisaidy; Aparicio-Ozores, Gerardo; Balcázar, José Luis; Eslava-Campos, Carlos Alberto; Hernández-Chiñas, Ulises; Molina-López, José.
Afiliação
  • González-Villalobos E; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Prolongación de Carpio y Plan de Ayala s/n, Colonia Santo Tomás, C.P. 11340, Mexico City, Mexico.
  • Ribas-Aparicio RM; Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas, Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, C.P. 04510, Mexico City, Mexico.
  • Montealegre GER; Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato-Oncología e Investigación, Hospital Infantil de México Federico Gómez/Facultad de Medicina, UNAM, Dr. Márquez 162 Col. Doctores. Alcaldía Cuauhtémoc, C.P. 06720, Mexico City, Mexico.
  • Belmont-Monroy L; Catalan Institute for Water Research (ICRA), 17003, Girona, Spain.
  • Ortega-García Y; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Prolongación de Carpio y Plan de Ayala s/n, Colonia Santo Tomás, C.P. 11340, Mexico City, Mexico.
  • Aparicio-Ozores G; Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas, Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, C.P. 04510, Mexico City, Mexico.
  • Balcázar JL; Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato-Oncología e Investigación, Hospital Infantil de México Federico Gómez/Facultad de Medicina, UNAM, Dr. Márquez 162 Col. Doctores. Alcaldía Cuauhtémoc, C.P. 06720, Mexico City, Mexico.
  • Eslava-Campos CA; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas (ENCB), Instituto Politécnico Nacional (IPN), Prolongación de Carpio y Plan de Ayala s/n, Colonia Santo Tomás, C.P. 11340, Mexico City, Mexico.
  • Hernández-Chiñas U; Unidad Periférica de Investigación Básica y Clínica en Enfermedades Infecciosas, Departamento de Salud Pública/División de Investigación, Facultad de Medicina, UNAM, C.P. 04510, Mexico City, Mexico.
  • Molina-López J; Laboratorio de Patogenicidad Bacteriana, Unidad de Hemato-Oncología e Investigación, Hospital Infantil de México Federico Gómez/Facultad de Medicina, UNAM, Dr. Márquez 162 Col. Doctores. Alcaldía Cuauhtémoc, C.P. 06720, Mexico City, Mexico.
Appl Microbiol Biotechnol ; 105(13): 5617-5629, 2021 Jul.
Article em En | MEDLINE | ID: mdl-34254156
Urinary tract infections (UTIs) are mainly caused by uropathogenic Escherichia coli (UPEC), whose impact can be exacerbated by multidrug-resistant (MDR) strains. Effective control strategies are, therefore, urgently needed. Among them, phage therapy represents a suitable alternative. Here, we describe the isolation and characterization of novel phages from wastewater samples, as well as their lytic activity against biofilm and adherence of UPEC to HEp-2 cells. The results demonstrated that phage vB_EcoM-phiEc1 (ϕEc1) belongs to Myoviridae family, whereas vB_EcoS-phiEc3 (ϕEc3) and vB_EcoS-phiEc4 (ϕEc4) belong to Siphoviridae family. Phages showed lytic activity against UPEC and gut commensal strains. Phage ϕEc1 lysed UPEC serogroups, whereas phages ϕEc3 and ϕEc4 lysed only UTI strains with higher prevalence toward the O25 serogroup. Moreover, phages ϕEc1 and ϕEc3 decreased both biofilm formation and adherence, whereas ϕEc4 was able to decrease adherence but not biofilm formation. In conclusion, these novel phages showed the ability to decrease biofilm and bacterial adherence, making them promising candidates for effective adjuvant treatment against UTIs caused by MDR UPEC strains. KEY POINTS: Phage with lytic activity against MDR UPEC strains were isolated and characterized under in vitro conditions. A novel method was proposed to evaluate phage activity against bacterial adherence in HEp-2 cell.. Phages represent a suitable strategy to control infections caused by MDR bacteria.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacteriófagos / Infecções Urinárias / Infecções por Escherichia coli / Escherichia coli Uropatogênica / Terapia por Fagos Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacteriófagos / Infecções Urinárias / Infecções por Escherichia coli / Escherichia coli Uropatogênica / Terapia por Fagos Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Appl Microbiol Biotechnol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha