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Detecting cancer cells with a highly sensitive LbL-based biosensor.
Correia, Abilene Rodrigues; Sampaio, Isabella; Comparetti, Edson José; Vieira, Nirton Cristi Silva; Zucolotto, Valtencir.
Afiliação
  • Correia AR; GNano - Nanomedicine and Nanotoxicology Group, São Carlos Institute of Physics, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil.
  • Sampaio I; GNano - Nanomedicine and Nanotoxicology Group, São Carlos Institute of Physics, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil. Electronic address: isabellasampaio@ymail.com.
  • Comparetti EJ; GNano - Nanomedicine and Nanotoxicology Group, São Carlos Institute of Physics, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil.
  • Vieira NCS; GNano - Nanomedicine and Nanotoxicology Group, São Carlos Institute of Physics, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil; Institute of Science and Technology, Federal University of São Paulo, 12231-280, São José dos Campos, SP, Brazil.
  • Zucolotto V; GNano - Nanomedicine and Nanotoxicology Group, São Carlos Institute of Physics, University of São Paulo, CP 369, 13560-970, São Carlos, SP, Brazil.
Talanta ; 233: 122506, 2021 Oct 01.
Article em En | MEDLINE | ID: mdl-34215121
Early diagnosis of cancer is crucial for therapeutic methods to be more effective and to decrease the mortality rate due to this disease. Current diagnostic methods include imaging techniques that require expensive equipment and specialized personnel, making it difficult to apply them to many patients. To overcome these limitations, many biosensors have been developed to monitor cancer biomarkers. Here, we report on the electrochemical biosensor for selective detection of tumor cells using a simple and low-cost methodology. Layer-by-layer (LbL) self-assembly was used to modify indium tin oxide (ITO) electrodes with alternating layers of polyallylamine hydrochloride (PAH) and folic acid (FA), which binds to overexpressed folate receptors alpha (FRα) in tumor cells. The LbL-based biosensor showed high sensitivity in detecting cervical cancer cells (HeLa cells) using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A linear dependence with the logarithm cell concentration was observed and excellent detection limits were found, 4 cells mL-1 and 19 cells mL-1 for EIS and CV measurements, respectively. The developed biosensor also presented great reproducibility (RSD = 1.7%) and repeatability (RSD = 1.8%). The selectivity was confirmed after the biosensor interaction with healthy cells (HMEC cells), which did not produce significant changes in the electrochemical signals. Furthermore, it was demonstrated that selective detection of tumor cells occurs via an interaction with FA. The LbL-based biosensor provides a simple, accurate, and cost-effective platform to be applied in the early diagnosis of cancer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Neoplasias Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Neoplasias Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda